LL10000 hybridization
Ontology highlight
ABSTRACT: The screening of a previously reported fluorescein labelled 10,000 member PNA encoded peptide library allowed information on the interaction between the peptide-ligands and the cell surface receptors to be extracted, identified new peptide ligands for cell surface receptors, and gave crucial information about consensus sequences. A novel indirect amplification of the PNA signal by amplification of the PNA-complementary DNA library was developed to screen PNA-encoded peptide library against D54, HEK293T, and HEK293T-CCR6 cells. This work generates a new approach to biological discovery and an expansion of modern microarray techniques. In addition, the microarray approach facilitates screening for differences in surface-receptor ligands and/or receptor expression between various cell types including diseased and normal cells.
ORGANISM(S): Homo sapiens
SUBMITTER: Nina Svensen
PROVIDER: E-MEXP-3104 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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