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LL10000 hybridization

ABSTRACT: The screening of a previously reported fluorescein labelled 10,000 member PNA encoded peptide library allowed information on the interaction between the peptide-ligands and the cell surface receptors to be extracted, identified new peptide ligands for cell surface receptors, and gave crucial information about consensus sequences. A novel indirect amplification of the PNA signal by amplification of the PNA-complementary DNA library was developed to screen PNA-encoded peptide library against D54, HEK293T, and HEK293T-CCR6 cells. This work generates a new approach to biological discovery and an expansion of modern microarray techniques. In addition, the microarray approach facilitates screening for differences in surface-receptor ligands and/or receptor expression between various cell types including diseased and normal cells.

ORGANISM(S): Homo sapiens

SUBMITTER: Nina Svensen

PROVIDER: E-MEXP-3104 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Encoded peptide libraries and the discovery of new cell binding ligands.

Svensen Nina N Díaz-Mochón Juan José JJ Bradley Mark M

Chemical communications (Cambridge, England) 20110609 27

Cells over-expressing integrins or CCR6 were incubated on a DNA microarray, pre-hybridized with a 10,000 member PNA-encoded peptide library allowing novel cell specific ligands for integrins and CCR6 to be identified. ...[more]

PMID: 21655632

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