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Transcriptomic analysis of differential genetic expression under CosR-knockdown condition compared with wild-type

ABSTRACT: We perfomed the transcriptomic analysis of differential genetic expression in the CosR-knockdown condition with a DNA microarray. CosR-specific antisense-PNA was used for CosR-knockdown. The results revealed that CosR significantly affected the expression of several genes involved in various cellular function in Campylobacter. Final goal of this study is to figure out the importance and the role of CosR in Campylobacter by identification of the CosR regulons. Two-condition experiment, WT vs. CosR-knockdown. Biological replicates: 3 wild type (control) replicates, 3 CosR-knockdown replicates. For preparing the total RNA, C. jejuni cells were grown at 42M-BM-0C in MH broth supplemented with 1.5 M-NM-

ORGANISM(S): Campylobacter jejuni

SUBMITTER: Sunyoung Hwang

PROVIDER: E-GEOD-40201 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional regulation of the CmeABC multidrug efflux pump and the KatA catalase by CosR in Campylobacter jejuni.

Hwang Sunyoung S Zhang Qijing Q Ryu Sangryeol S Jeon Byeonghwa B

Journal of bacteriology 20121012 24

CosR is an essential response regulator in Campylobacter jejuni, a major food-borne pathogen causing enteritis worldwide. A transcriptomic analysis performed in this study discovered 93 genes whose transcriptional levels were changed >2-fold due to the repression of CosR expression by antisense peptide nucleic acid. The identified CosR-regulated genes are involved in various cellular functions, such as energy production, protein synthesis and folding, flagellum biogenesis, and lipid metabolism. ...[more]

PMID: 23065977

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