Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of B-cells stimulated by different compounds (CIDR GST anti Ig)

ABSTRACT: Transcription profiling of B-cells stimulated by different compunds (CIDR; GST; anti Ig)

ORGANISM(S): Homo sapiens

SUBMITTER: Bobo Mok

PROVIDER: E-MEXP-316 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Increased B cell survival and preferential activation of the memory compartment by a malaria polyclonal B cell activator.

Donati Daria D Mok Bobo B Chêne Arnaud A Xu Hong H Thangarajh Mathula M Glas Rickard R Chen Qijun Q Wahlgren Mats M Bejarano Maria Teresa MT

Journal of immunology (Baltimore, Md. : 1950) 20060901 5

Chronic malaria infection is characterized by polyclonal B cell activation, hyperglobulinemia, and elevated titers of autoantibodies. We have recently identified the cysteine-rich interdomain region 1alpha (CIDR1alpha) of the Plasmodium falciparum erythrocyte membrane protein 1 as a T cell-independent polyclonal B cell activator and Ig binding protein. Here, we show that, although the binding affinity of CIDR1alpha to human IgM and IgG is relatively low, B cell activation still proceeds. CIDR1al ...[more]

PMID: 16920940

Similar Datasets

Understanding the physiological role of asparaginyl endopeptidase (AEP)/Legumain

Project description:Understanding the regulation of lysosomal proteolytic/hidrolytic capacity has been recently advanced in a huge manner by the discovery of the lysosome-mTOR-TFEB pathway connecting nutrient sensing, autophagy and de novo lysosome generation. But, how lysosomes can adjust their proteolytic/hidrolytic capacity to meet varying conditions (more or less substrate) under normal fed conditions is not known. We have used AEP as a proxy to understand how lysosomes can compensate the lack of a key lysosomal protease to meet the requirements under physiological, fed conditions. To do this, we did compare cytoplasmic, membrane and nuclear fractions of WT and AEP MEFs that were expanded in SILAC media. AEP knock-out mice show pale kidney, with abnormal proliferation of the epithelial proximal tubular cells. In an attempt to understand the role of AEP in the kidney, we did compare kidney obtained from WT and AEP mice using kidneys from WT mice that were fed with SILAC food. Also, in an attempt to reproduce the changes observed in the absence of AEP in kidney and MEFs, we treated WT MEFs grown in SILAC media with MVO26630, specific inhibitor of AEP, for 12 and 48 hours.

2019-01-09 | PXD011473 | Pride

Transcription profiling by array of TMPAP viral stable transfected LNCaP cells

Project description:To determine the effects produced by the overexpression of transmembrane prostatic acid phosphatase (TMPAP) in viral stable transfected LNCaP cells.

2015-10-02 | E-MTAB-2730 | biostudies-arrayexpress

Transcription profiling by array of human B cell lymphoma cell lines in response to rituximab treatment

Project description:HF-1, Granta-519, SUDHL4,Oci-Ly10 and Oci-Ly3 cell lines, representing different B cell lymphoma subtypes, were induced with rituximab (R) for three hours and analyzed for differentially expressed genes compared to untreated control.

2010-06-22 | E-MEXP-2317 | biostudies-arrayexpress

Characterization of the miRNA profile of activated or apoptotic lymphocytes and corresponding released microvesicles in normal healthy donors and systemic lupus erythematosus (SLE) patients.

Project description:In this experiment, the miRNA profile of microvesicles (MV) released from activated or apoptotic (UV-B treated) T-lymphocytes and their cells of origin was analyzed in both samples obtained from normal healthy donors and systemic lupus erythematosus (SLE) patients. Therefore in short, RNA was isolated of activated and apoptotic (UV-B treated) T-Lymphocytes and corresponding MV of normal healthy individuals and systemic lupus erythematosus (SLE) patients. Samples of four donors each were pooled. The aim was to characterize the miRNA profile of MV dependent on different release stimuli and compare their miRNA-profile to their cells of origin.

2018-03-31 | E-MTAB-3944 | biostudies-arrayexpress

Genome-Wide Analysis of Somatic Copy Number Alterations (CGH, SNP) and Gene Expression (RNA-seq) in Metastatic Melanoma

Project description:All cancers are diseases of the genome. A combination of somatic point mutations, focal amplifications and deletions, and chromosome level aberrations conspire to disrupt gene expression and the interplay between signaling pathways that control normal growth and tissue homeostasis. Here we investigate somatic copy number abberations in metastatic melanomas. A metastatic melanoma was assayed on Affymetrix SNP arrays to detect copy number abberations. 7 cutaneous melanomas as well as their matched control ( peripheral blood lymphocytes (PBL) or Epstein-Barr virus transformed lymphoblastoid cell lines ) and 2 control melanocytes were assayed on Illumina SNP arrays. 7 metastatic melanomas were hybridized on Agilent CGH arrays using donor matched control as reference.

2011-04-07 | E-GEOD-22928 | biostudies-arrayexpress

Systems analysis identifies an essential role for SHARPIN in macrophage TLR2 responses (Affymetrix)

Project description:Our systems analysis reported here demonstrates that TLR-responses in macrophages are markedly impaired by SHARPIN deficiency, and that SHARPIN controls expression of a subset of TLR2-induced, NF-kB and AP-1 dependent genes that overlaps with those affected by the hypomorphic panr2 mutation in NEMO. 46 total RNA samples from murine bone marrow derived macrophages were analyzed (32 by Agilent array, 14 by Affymetrix Exon array) On Affymetrix Exon array, the responses of macrophages to 12hr stimulation with the TLR2 ligand PAM3CSK4 (300ng/mL) were analyzed in biological duplicates for the Map3k8(sluggish) mutant and respective controls and in singlet for the Tnf(KO) mutant compared to two respective WT controls.

2011-08-30 | E-GEOD-29891 | biostudies-arrayexpress

Transcription profiling of wild type and ATF3 -/-mouse bone marrow macrophages stimulated with lipopolysaccharide for 1, 2, 4, 8 and 24 hours

Project description:Time course data from lipopolysaccharide-stimulated wild-type and ATF3 -/- mouse bone marrow macrophages were collected to investigate the transcriptional network in Toll-like recepter 4-activated macrophages.

2006-06-08 | E-TABM-102 | biostudies-arrayexpress

Systems analysis identifies an essential role for SHARPIN in macrophage TLR2 responses (Agilent)

Project description:Our systems analysis reported here demonstrates that TLR-responses in macrophages are markedly impaired by SHARPIN deficiency, and that SHARPIN controls expression of a subset of TLR2-induced, NF-kB and AP-1 dependent genes that overlaps with those affected by the hypomorphic panr2 mutation in NEMO. 46 total RNA samples from murine bone marrow derived macrophages were analyzed (32 by Agilent array, 14 by Affymetrix Exon array) On Agilent array, the responses of macrophages to 12hr stimulation with the TLR2 ligand PAM3CSK4 (300ng/mL) were analyzed in biological duplicates for five mutant mouse strains and respective controls: Sharpin(cpdm), Ikbkg(panr2), Atf3(KO), Il10(KO), and Nfkb1(KO).

2011-08-30 | E-GEOD-29888 | biostudies-arrayexpress

Single-cell RNA-seq of ex vivo CD45-negative skin resident-cells using microfluidic droplet capture

Project description:To obtain a large representation of non-immune skin-resident cells, we profiled CD45-negative cells from a digested skin sample taken from a human female

2018-07-06 | E-MTAB-6831 | biostudies-arrayexpress

Genome-Wide Analysis of Somatic Copy Number Alterations and Gene Expression in Metastatic Melanoma

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2011-04-07 | E-GEOD-23056 | biostudies-arrayexpress