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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptome profiling of irg1 mutant leaf of Medicago truncatula

ABSTRACT: To gain a molecular understanding of the loss-of-function mutation of a transcription factor involved in leaf morphogenesis impacts symmetric cuticular wax loading in M. truncatula, we compared the transcript profiles of wild-type R108 and three independent irg1/palm1 homozygous null mutant lines (irg1-1, irg1-2, and irg1-5).

ORGANISM(S): Medicago truncatula

SUBMITTER: Yuhong Tang

PROVIDER: E-MEXP-3190 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Loss of abaxial leaf epicuticular wax in Medicago truncatula irg1/palm1 mutants results in reduced spore differentiation of anthracnose and nonhost rust pathogens.

Uppalapati Srinivasa Rao SR Ishiga Yasuhiro Y Doraiswamy Vanthana V Bedair Mohamed M Mittal Shipra S Chen Jianghua J Nakashima Jin J Tang Yuhong Y Tadege Million M Ratet Pascal P Chen Rujin R Schultheiss Holger H Mysore Kirankumar S KS

The Plant cell 20120131 1

To identify genes that confer nonhost resistance to biotrophic fungal pathogens, we did a forward-genetics screen using Medicago truncatula Tnt1 retrotransposon insertion lines. From this screen, we identified an inhibitor of rust germ tube differentation1 (irg1) mutant that failed to promote preinfection structure differentiation of two rust pathogens, Phakopsora pachyrhizi and Puccinia emaculata, and one anthracnose pathogen, Colletotrichum trifolii, on the abaxial leaf surface. Cytological an ...[more]

PMID: 22294617

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