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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of CD4+ T lymphocytes in response to an irradiation dose of 1 Gy.radiation

ABSTRACT: Differences in radiosensitivity existing among cells which constitute the lymphocyte population have been known for a long time, although the molecular basis of this differential radiation sensitivity remains unclear. In an attempt to get more insight into the molecular mechanisms leading to radiation effects, we used microarray analysis to study transcriptional changes in CD4+ T lymphocytes to an irradiation dose of 1 Gy. The contribution of the cell type to the radiation response of CD4+ lymphocytes was deduced by comparing our results with a gene expression profile observed in Peripheral Blood Lymphocytes published by other groups (Amundson, 2000; Kang, 2003, Jen, 2003).

ORGANISM(S): Homo sapiens

SUBMITTER: M. Benotmane

PROVIDER: E-MEXP-320 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional response to ionizing radiation in lymphocyte subsets.

Mori M M Benotmane M A MA Tirone I I Hooghe-Peters E L EL Desaintes C C

Cellular and molecular life sciences : CMLS 20050701 13

Human lymphocyte subpopulations differ in their cellular responses to ionizing radiation. To shed light on the molecular basis of this effect, we characterized the transcriptional response to 1 Gy X-rays of CD4+ T lymphocytes. Of 18,433 genes tested, 102 were modulated more than 1.5-fold. The majority of the strongly activated genes were p53 targets involved in DNA repair and apoptosis. The expression of three of these genes was further tested by quantitative RT-PCR in lymphocyte subpopulations ...[more]

PMID: 15971001

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