Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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KT2440 wt vs. mut agmR -M9 plus glucose chloramphenicol-free medium

ABSTRACT: DNA microarrays were carried out to compare the transcriptional profiles of P. putida KT2440 and its isogenic knock-out mutant in agmR gene (mut agmR) when grown in M9 medium with glucose as a sole carbon source and in absense of chloramphenicol or any other stressor compound.

ORGANISM(S): Pseudomonas putida

SUBMITTER: Matilde Fernandez 

PROVIDER: E-MEXP-3203 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Mechanisms of resistance to chloramphenicol in Pseudomonas putida KT2440.

Fernández Matilde M   Conde Susana S   de la Torre Jesús J   Molina-Santiago Carlos C   Ramos Juan-Luis JL   Duque Estrella E  

Antimicrobial agents and chemotherapy 20111205 2

Pseudomonas putida KT2440 is a chloramphenicol-resistant bacterium that is able to grow in the presence of this antibiotic at a concentration of up to 25 μg/ml. Transcriptomic analyses revealed that the expression profile of 102 genes changed in response to this concentration of chloramphenicol in the culture medium. The genes that showed altered expression include those involved in general metabolism, cellular stress response, gene regulation, efflux pump transporters, and protein biosynthesis.  ...[more]

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