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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptomic characterization of a synergistic genetic interaction during carpel margin meristem development

ABSTRACT: We were testing the expression differences of seuss, aintegumenta, and double mutants in stage 8-10 dissected carpels from Arabidopsis thaliana.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: April Wynn

PROVIDER: E-MEXP-3293 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptomic characterization of a synergistic genetic interaction during carpel margin meristem development in Arabidopsis thaliana.

Wynn April N AN Rueschhoff Elizabeth E EE Franks Robert G RG

PloS one 20111021 10

In flowering plants the gynoecium is the female reproductive structure. In Arabidopsis thaliana ovules initiate within the developing gynoecium from meristematic tissue located along the margins of the floral carpels. When fertilized the ovules will develop into seeds. SEUSS (SEU) and AINTEGUMENTA (ANT) encode transcriptional regulators that are critical for the proper formation of ovules from the carpel margin meristem (CMM). The synergistic loss of ovule initiation observed in the seu ant doub ...[more]

PMID: 22031826

Similar Datasets

Transcriptomic characterization of a synergistic genetic interaction during carpel margin meristem development

Project description:We are interested in examining the transcriptional hierarchies that are required for proper formation and function of the carpel margin meristem in Arabidopsis thaliana. The carpel margin meristem (CMM) gives rise to the ovule primorida (which begin to form around flower stage 8). We know that the loss of the seuss-3 (seu-3) and aintegumenta-1(ant-1) separately leads to a mild disruption of ovule formation, while the loss of both causes a complete loss of ovule formation. In this study we wanted to examine expression levels changed between each single mutant and the double mutant. We dissected out stage 8-10 carpel of each of the four genotypes (seu-3, ant-1, Col-0, seu-3 ant-1) pooling 15-25 carpels into one biological replicate. Then using 16 ATH1 arrays we hybridized, amplified and labeled cDNA to 16 arrays (4 replicates for each genotype). We then analyzed our data to reveal transcripts that went down in the double compared to either single mutant, and transcripts that went down more than additively in the double mutant compared to added level of the decrease of the single mutant levels. 16 arrays: 4 seuss-3, 4 aintegumenta-1, 4 Col-0, 4 seuss-3 aintegumenta-1. Each biological replicate was a sample of 15-25 pooled stage 8-10 carpels manually dissected by hand.

2011-07-31 | E-GEOD-30492 | biostudies-arrayexpress

Transcriptomic characterization of a synergistic genetic interaction during carpel margin meristem development in Arabidopsis thaliana

2011-08-01 | GSE30492 | GEO

Transcriptomic characterization of a synergistic genetic interaction during carpel margin meristem development in Arabidopsis thaliana.

Project description:In flowering plants the gynoecium is the female reproductive structure. In Arabidopsis thaliana ovules initiate within the developing gynoecium from meristematic tissue located along the margins of the floral carpels. When fertilized the ovules will develop into seeds. SEUSS (SEU) and AINTEGUMENTA (ANT) encode transcriptional regulators that are critical for the proper formation of ovules from the carpel margin meristem (CMM). The synergistic loss of ovule initiation observed in the seu ant double mutant suggests that SEU and ANT share overlapping functions during CMM development. However the molecular mechanism underlying this synergistic interaction is unknown. Using the ATH1 transcriptomics platform we identified transcripts that were differentially expressed in seu ant double mutant relative to wild type and single mutant gynoecia. In particular we sought to identify transcripts whose expression was dependent on the coordinated activities of the SEU and ANT gene products. Our analysis identifies a diverse set of transcripts that display altered expression in the seu ant double mutant tissues. The analysis of overrepresented Gene Ontology classifications suggests a preponderance of transcriptional regulators including multiple members of the REPRODUCTIVE MERISTEMS (REM) and GROWTH-REGULATING FACTOR (GRF) families are mis-regulated in the seu ant gynoecia. Our in situ hybridization analyses indicate that many of these genes are preferentially expressed within the developing CMM. This study is the first step toward a detailed description of the transcriptional regulatory hierarchies that control the development of the CMM and ovule initiation. Understanding the regulatory hierarchy controlled by SEU and ANT will clarify the molecular mechanism of the functional redundancy of these two genes and illuminate the developmental and molecular events required for CMM development and ovule initiation.

| S-EPMC3198736 | biostudies-literature

Transcription profiling of pistils from Arabidopsis wild type, ant-4 mutant and ino-1mutant plants at different developmental stages

Project description:This experiment was designed to identify genes expressed preferentially in the two integuments of the Arabidopsis ovule. Pistils from wild type and two ovule mutants were compared against each aintegumenta-4 (ant-4) which lacks both integuments and inner no outer (ino-1) which lacks the outer integument. Genes that are highly expressed only in the integuments were expected to be reduced in expression in the mutants, as compared with wild type. Pistils containing ovules through all stages of ovule development prior to pollination were pooled for one experiment (FULL arrays), and for two separate experiments, a set of early differentiation stages (EARLY arrays) and a set of later differentiation stages (LATE ARRAYS) were pooled. Wild type and mutant lines are in the ecotype Landsberg erecta.

2009-06-01 | E-MEXP-1920 | biostudies-arrayexpress

The CUC1 and CUC2 genes promote carpel margin meristem formation during Arabidopsis gynoecium development.

Project description:Carpel margin meristems (CMMs), a pair of meristematic tissues present along the margins of two fused carpel primordia of Arabidopsis thaliana, are essential for the formation of ovules and the septum, two major internal structures of the gynoecium. Although a number of regulatory factors involved in shoot meristem activity are known to be required for the formation of these gynoecial structures, their direct roles in CMM development have yet to be addressed. Here we show that the CUP-SHAPED COTYLEDON genes CUC1 and CUC2, which are essential for shoot meristem initiation, are also required for formation and stable positioning of the CMMs. Early in CMM formation, CUC1 and CUC2 are also required for expression of the SHOOT MERISTEMLESS gene, a central regulator for stem cell maintenance in the shoot meristem. Moreover, plants carrying miR164-resistant forms of CUC1 and CUC2 resulted in extra CMM activity with altered positioning. Our results thus demonstrate that the two regulatory proteins controlling shoot meristem activity also play critical roles in elaboration of the female reproductive organ through the control of meristematic activity.

| S-EPMC4012194 | biostudies-literature

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Project description:Culture of proB cells with IL7 in the presence or absence of CpG

2010-02-27 | E-MEXP-2595 | biostudies-arrayexpress

Transcription profiling of yeast sum1 delta strain at three time points after the start of meiosis

Project description:Expression profiling of sum1 delta strain at three time points after the start of meiosis

2010-04-20 | E-MEXP-2154 | biostudies-arrayexpress

Transcription profiling of yeast during meiosis in a strain with Ndt80 induced with estradiol

Project description:Expression profile of meiosis in strain with Ndt80 inducible by addition of Estradiol (as in Carlile and Amon 2008), in three time points from addition of Estradiol.

2010-04-20 | E-MEXP-2155 | biostudies-arrayexpress

Transcription profiling of yeast W303 cells at multiple time points during meiosis

Project description:expression profiling of meiotic W303 cells in duplicate- multiple time points

2009-07-28 | E-MEXP-1781 | biostudies-arrayexpress

Transcription profiling of yeast pdr mutants

Project description:PDR mutants , strain YYA100 (pdr1?::KanMx6, pdr3?::His3Mx6) from Mol Biol Cell. 2007 December; 18(12): 49324944, was grown to OD 1-1.5 in YEPD and hybridyzed to an expression array in one channel with a wild-type strain reference RNA on the other channel

2009-06-17 | E-MEXP-2150 | biostudies-arrayexpress

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