Sex Biased Gene Expression in Mate-Seeking Anopheles gambiae M and S

ABSTRACT: Because premating behavioral isolation typically involves the interaction between traits in males and females, we generated whole-genome expression profiles of virgin males collected at the same age and diel time as the virgin females assayed in Cassone et al. (2010). Using the same Affymetrix Anopheles/Plasmodian GeneChip platform, levels and patterns of transcript abundance were examined between lab colonies of M and S Quantitative real-time PCR (qRT-PCR) was used to examine tissue-specific expression of candidate genes, as well as to validate transcription variation using RNA samples of M and S mosquitoes collected from natural populations in Burkina Faso and Cameroon. Gas chromatography-mass spectrometry was performed to examine qualitative differences in virgin male cuticular hydrocarbon composition between forms at mid-dusk and two additional time points.

ORGANISM(S): Anopheles gambiae

SUBMITTER: Bryan Cassone 

PROVIDER: E-MEXP-3300 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress