Effect of mifepristone on gene expression in Drosophila
Ontology highlight
ABSTRACT:
ORGANISM(S): Drosophila melanogaster
PROVIDER: GSE64474 | GEO | 2015/01/18
SECONDARY ACCESSION(S): PRJNA271084
REPOSITORIES: GEO
Similar Datasets
Project description:Illumina sequencing was used to assay the effect of mifepristone treatment on gene expression in adult Drosophila, including males, virgin females and mated females. Males of strain w[1118]; p53B[6] were crossed to virgins of w[1118]; rtTA(3)E2 and progeny males and virgins were collected over 48 hours. One half of the virgins were mated to w[1118] males at ratio of 1:1 virgins to males for 4 days. Mated females were then separated from the w[1118] males. The mated females, males and virgins females were then maintained at approximately 20 flies per vial, on food with and without supplementation with 160ug/ml mifepristone for 12 days. Total fly RNA was isolated from 20 animals per sample. Three replicate samples were generated for each type of flies: males, mated females and virgin females.
2015-01-18 | E-GEOD-64474 | biostudies-arrayexpress
Project description:Comparison of gene expression between females that were either virgin, mated to normal males, mated to spermless males (sons of tudor) or mated to sperm-less/Acp-less males (DTA-E). Keywords: other
2004-12-09 | GSE1507 | GEO
Project description:Assessment of the seminal fluid proteins of Drosophila mojavensis and Drosophila arizonae. Experiment was performed using SILAC, whereas D. arizonae males were labeled with L-lysine-2HCL, 4,4,5,5-D4 (Lys 4) and D. mojavensis males labeled with L-Lysine-13C6,15N2 (Lys 8) and mated to their respective conspecific females (unlabeled). Following copulation females were immediately frozen in liquid nitrogen and stored at -80 C until reproductive tracts were removed and placed in 50 mM ammonium bicarbonate.
2024-06-19 | PXD041260 | Pride
Project description:Small RNA sequencing from Drosophila pseudoobscura males and virgin females.
2021-07-14 | GSE179989 | GEO
Project description:Comparison of gene expression between females that were either virgin, mated to normal males, mated to spermless males (sons of tudor) or mated to sperm-less/Acp-less males (DTA-E).
2008-06-11 | E-GEOD-1507 | biostudies-arrayexpress
Project description:Sex Peptide, a seminal fluid protein of Drosophila melanogaster males, elicits an array of post-mating responses in females, including decreased receptivity to re-mating, and increased egg laying, activity and food intake, with a preference for protein-rich food. To determine how one protein can have such widespread effects, we set out to dissect the genetic architecture of the female’s response to Sex Peptide, to determine whether Sex Peptide alters the expression of several regulators targeted to specific post-mating responses or acts on a pleiotropic regulator that controls multiple responses. We performed bulk RNA-seq of female heads at 10 time points within the first 24 hours after mating, sampling virgin females, females mated to control males and females mated to Sex Peptide-null males. Using this high-resolution time series, we identified mating- and Sex Peptide-dependent differentially expressed genes and discovered the presence of differentially used exons. We constructed gene regulatory networks using clustering and motif enrichment analyses, and identified cell types in which these changes might take place using deconvolution of our bulk RNA-seq dataset. One key network included metabolic genes which might change in expression in the female’s fat body. A second network included genes with neuronal functions, whose changes might be located in neurons or sensory organs in the female’s head. Within these networks we identified known molecular regulators of the circadian clock. Further, we found that many differentially expressed genes, and some differentially used exons, followed a circadian rhythm in virgin females, and that this rhythm was altered after mating with a Sex Peptide- male.
2023-01-27 | GSE198879 | GEO
Project description:This SuperSeries is composed of the following subset Series: GSE19571: Ceratitis capitata gene expression related to sexual maturation in females GSE19572: Ceratitis capitata gene expression related to sexual maturation in males GSE19573: Ceratitis capitata gene expression related to mating status in mature females (mated vs virgin) GSE19608: Ceratitis capitata gene expression related to mating status in mature males (mated vs virgin) Refer to individual Series
2011-12-25 | E-GEOD-19614 | biostudies-arrayexpress
Project description:In this analysis, we used microarrays to contrast genome-wide transcript levels in virgin versus mated females before and after infection. We repeated the entire experiment using female mutants that do not form a germline. We found that multiple genes involved in egg production show reduced expression in response to infection, and that this reduction is stronger in virgins than it is in mated females. In germline-less females, expression of egg-production genes was predictably low and not differentially affected by infection. We also identified several immune responsive genes that are differentially induced after infection in virgins versus mated females.
2013-03-19 | GSE45296 | GEO
Project description:We present data using a novel method to simultaneously identify and quantify transferred male seminal proteins and the female reproductive proteome using multiplexed Tandem-Mass-Tag (TMT) isobaric labelling of the lower female reproductive tracts dissected from virgin- or recently mated- females of three species of the virilis group. We identified over 200 putative male ejaculate proteins many of which show differential abundance between species. We also identified over 2000 proteins providing the first description of the Drosophila female reproductive tract proteome outside of the melanogaster group which also shows significant divergence between species. We then assessed the utility of species-specific compared to single species query databases for protein identification and quantification.
2023-10-24 | PXD031638 | Pride
Project description:In Drosophila melanogaster, mating radically transforms female physiology and behavior. Post-mating responses include an increase in the oviposition rate, a reduction in female receptivity, and an activation of the immune system . The fitness consequences of mating are similarly dramatic – females must mate once in order to produce fertile eggs, but additional matings have a clear negative effect. Previously, microarrays have been used to examine gene expression of females differing in their reproductive status with the aim of identifying genes influenced by mating. However, since only virgin and single mated females were compared, transcriptional changes associated with reproduction (under natural selection) and the effects of male-induced harm (under sexually antagonistic selection) cannot be disentangled. We partitioned these fundamentally different effects by instead examining the expression profiles of virgin, single mated and double mated females. We found substantial effects relating to reproduction and further effects that are only attributable to a second mating. Immune response genes dominate this male-induced harm effect indicating that the cost of mating may be due partly to this system's activation. We propose that both sexually antagonistic and natural selection have been important in the evolution of the innate immunity genes, thereby contributing to the sexual dimorphismand rapid evolution at these loci. Keywords: Female response to mating
2009-02-19 | GSE12834 | GEO