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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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90 min Met4 hyperactivation ChIP-chip

ABSTRACT: Identification of in vivo binding targets of Met4 and Met32 by conducting chromatin immunoprecipitation studies using genomic tiling arrays (ChIP-on-chip 4X44K whole yeast genome arrays by Agilent) following 90 minutes Met4 hyperactivation.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Traci Lee

PROVIDER: E-MEXP-3302 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Characterizing the roles of Met31 and Met32 in coordinating Met4-activated transcription in the absence of Met30.

Carrillo Emilio E Ben-Ari Giora G Wildenhain Jan J Tyers Mike M Grammentz Dilon D Lee Traci A TA

Molecular biology of the cell 20120321 10

Yeast sulfur metabolism is transcriptionally regulated by the activator Met4. Met4 lacks DNA-binding ability and relies on interactions with Met31 and Met32, paralogous proteins that bind the same cis-regulatory element, to activate its targets. Although Met31 and Met32 are redundant for growth in the absence of methionine, studies indicate that Met32 has a prominent role over Met31 when Met30, a negative regulator of Met4 and Met32, is inactive. To characterize different roles of Met31 and Met3 ...[more]

PMID: 22438580

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