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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Hematopoietic progenitors from differentiating RUNX1cGFP human embryonic stem cells

ABSTRACT: In order to identify cells expressing RUNX1c during hematopoietic differentiation of human embryonic stem cells (hESCs), we targeted GFP downstream of the RUNX1 distal promoter. GFP was observed from 10-25 days of embryoid body differentiation and accurately mirrored expression of the endogenous RUNX1c isoform. GFP was restricted to CD45+ hematopoietic cells and GFP+CD34+ cells were highly enriched for progenitor cells. Appearance of the first wave of hematopoietic blast colony forming cells (Bl-CFC) in d3-4 embryoid bodies, antedated the expression of RUNX1c. These results confirm a role for RUNX1c in marking a second wave of hematopoietic progenitor cells in differentiating hESCs. The purpose of the cord blood samples was to perform comparison of them to the gene expression profiles of the Runx fractions - as the cord blood represents a heterogeneous mix of hematopoietic stem cells and multipotent progenitors cells.

ORGANISM(S): Homo sapiens

SUBMITTER: CLaire Hirst

PROVIDER: E-MEXP-3356 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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