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Trancriptional regulation by axonal SMN on motor neuron cells

ABSTRACT: Muscular atrophy (SMA) is an autosomal recessive disease causing selective motor neuron death by the loss of telomeric survival motor neuron gene, SMN1. Axonal SMN, a-SMN, is a truncated form of SMN, derived from an alternatively spliced SMN1 gene. (Setola, et. al. 2007 PNAS 104, 1959-1964). The cellular clones expressing a-SMN in a tetracycline-dependent manner were isolated from NSC34 by two-step stable transfection, first with the tetracycline-repressor construct and subsequently with the a-SMN cDNA. To identify novel a-SMN target genes, the transcriptome of several a-SMN clones was analyzed and compared with that of parental cells.

ORGANISM(S): Mus musculus

SUBMITTER: Maddalena Fratelli

PROVIDER: E-MEXP-3402 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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