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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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BIGenomics_Agilent_15k_templates

ABSTRACT: Hybridization of ligation probes to a zipcode (tag) microarray. The purpose of the experiment was to establish functionality and target specificity of the ligation probes by using pools of synthetic templates with all of the probes. The templates are 80-mer synthetic dsDNA molecules, each specific for a single probe. Each probe is a ssDNA molecule containing target recognition sequences, PCR primer binding sequences and a unique tag sequence. If a matching target sequence is present in the mixture, the probes is ligated into a circular molecule, which can be PCR amplified and hybrized on a microarray harbouring complementary tag sequences.

ORGANISM(S): bioreactor metagenome

SUBMITTER: Jarmo Ritari

PROVIDER: E-MEXP-3538 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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