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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression in the thymus medulla in the absence and presence of acute graft-versus-host disease

ABSTRACT: To investigate gene expression profile of medullary thymic epithelial cells with high surface density of MHC class II (mTEChigh), a murine parent into F1 hematopoietic stem cell transplantation model was administered. As recipient mice (B6xDBA/2)F1 (B6D2F1) were used that received either a syngeneic transplant from a (B6xDBA/2)F1 mouse or an allogeneic transplant from a B6 mouse, which leads to acute graft-versus-host disease (aGVHD). mTEChigh (CD45-EpCAM+Ly51-UEA1+MHCIIhigh) are sorted by FACS.

ORGANISM(S): Mus musculus

SUBMITTER: Simone Dertschnig

PROVIDER: E-MEXP-3745 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Epithelial cytoprotection sustains ectopic expression of tissue-restricted antigens in the thymus during murine acute GVHD.

Dertschnig Simone S Nusspaumer Gretel G Ivanek Robert R Hauri-Hohl Mathias M MM Holländer Georg A GA Krenger Werner W

Blood 20130529 5

Development of acute graft-versus-host disease (aGVHD) predisposes to chronic GVHD with autoimmune manifestations. A characteristic of experimental aGVHD is the de novo generation of autoreactive T cells. Central tolerance is dependent on the intrathymic expression of tissue-restricted peripheral self-antigens (TRA), which is in mature medullary thymic epithelial cells (mTEC(high)) partly controlled by the autoimmune regulator (Aire). Because TECs are targets of donor T-cell alloimmunity, we tes ...[more]

PMID: 23719300

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Leukocyte accumulation in graft blood vessels during self-limiting acute rejection of rat kidneys Immunobiology

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2010-09-24 | E-GEOD-18266 | biostudies-arrayexpress

Redefining thymus medulla specialization for central tolerance.

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promiscuous gene expression in the mouse thymus

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2005-04-28 | GSE2585 | GEO

The effect of cortex/medulla proportions on molecular diagnoses in kidney transplant biopsies: rejection and injury can be assessed in medulla

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