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Comparative genomic hybridization of Photbacterium profundum 3TCK genomic DNA vs P profundum SS9 genomic DNA

ABSTRACT: Genomic DNA extracted from two different Photobacterium profundum strains: SS9 strain (completely sequenced and used to made the microarray) and 3TCK strain were labeled with Cy3 and Cy5 fluorophores and competitively hybridized on the microarray built on the basis of the SS9 strain genomic sequence. Aim: the identification of the genomic regions absent in the 3TCK strain with respect to the SS9 strain. The SS9 strain was isolated from the Sulu Trench and displays an optimum growth at 28 MPa (2800 metres of depth). The 3TCK strain was isolated from a coastal sample collected near San Diego, California and has an optimum growth at 0.1 MPa.

ORGANISM(S): Photobacterium profundum

SUBMITTER: Stefano Campanaro 

PROVIDER: E-MEXP-375 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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<h4>Background</h4>Oceans cover approximately 70% of the Earth's surface with an average depth of 3800 m and a pressure of 38 MPa, thus a large part of the biosphere is occupied by high pressure environments. Piezophilic (pressure-loving) organisms are adapted to deep-sea life and grow optimally at pressures higher than 0.1 MPa. To better understand high pressure adaptation from a genomic point of view three different Photobacterium profundum strains were compared. Using the sequenced piezophile  ...[more]

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