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Transcription profiling of Yersinia pestis overexpressing phoP

ABSTRACT: Microarrays were used to identify Y. pestis genes that are differentially regulated under conditions of phoP overexpression. RNA samples were isolated from cultures of two isogenic Y. pestis strains, one overexpressing phoP (KIM10+phoP(delta)/PhoP) and the other not (KIM10+phoP(delta)/MMB67EH), and their gene expression profiles were compared.

INSTRUMENT(S): GenePix 4000B [Axon Instruments]

ORGANISM(S): Yersinia pestis

SUBMITTER: Lance Palmer

PROVIDER: E-MEXP-712 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Characterization of phagosome trafficking and identification of PhoP-regulated genes important for survival of Yersinia pestis in macrophages.

Grabenstein Jens P JP Fukuto Hana S HS Palmer Lance E LE Bliska James B JB

Infection and immunity 20060701 7

The transcriptional activator PhoP is important for survival of Yersinia pestis in macrophage phagosomes. However, the phagosomes inhabited by Y. pestis have not been well characterized, and the mechanism by which PhoP promotes bacterial survival in these vacuoles is not fully understood. Lysosomal tracers, as well as antibodies to late endosomal or lysosomal proteins, were used in conjunction with confocal or electron microscopy to study the trafficking of phagosomes containing phoP(+) or phoP ...[more]

PMID: 16790745

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