Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

89 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Glomerular endotelium and EPC MVs

ABSTRACT: Glomerular endothelial cells were cultured in normal condition and treated or not with microvesicles derived from endothelial progenitor cells. mRNA profiling of glomerular endothelial cells , treated with MVs, was analyzed after normalization with mRNA profiling of untreated cells.

ORGANISM(S): Homo sapiens

SUBMITTER: Vincenzo Cantaluppi

PROVIDER: E-MEXP-3762 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Hypoxic tubular endothelium and EPC MVs

Project description:Tubular endothelial cells were cultured in hypoxic conditions (1% O2) and treated or not with microvesicles derived from endothelial progenitor cells.<br>mRNA profiling of hypoxic cells, treated or not with MVs, was analyzed after nromalization with mRNA profiling of normoxic cells.<br><br>This experiment was updated on 27th Jan 2011 to correct descriptions.

2011-01-29 | E-MEXP-2972 | biostudies-arrayexpress

Apoptosis in normoxic and hypoxic tubular epithelial cells treated with EPC MVs

Project description:TECs cultured in normoxic conditions, TECs cultured in hypoxic conditions and TECs cultured in hypoxic treated with EPc derived microvesicles

2011-02-04 | E-MEXP-3086 | biostudies-arrayexpress

Transcription profiling by array of human islet endothelial cells (hIECs) stimulated with or without 50 ug/ml of islet-derived extracellular vesicles for 12 and 24 hours.

Project description:Human islet endothelial cells (hIECs) were stimulated or not with 50 ug/ml of islet-derived EVs for 12 and 24 hours.

2012-08-08 | E-MEXP-3693 | biostudies-arrayexpress

Huvec angiogenesis in hypoxic condition treated with caffeic acid

Project description:HUVECs were cultured in hypoxic conditions for 24 hours and treated or not with 1uM caffeic acid. Then cells were evaluated for expression of different genes involved in angiogenesis

2013-06-03 | E-MEXP-3908 | biostudies-arrayexpress

transcriptional profile of islet EVs for diabetes genes

Project description:Identification of transcriptional profile of several genes involved in diabetes in islet-derived extracellular vesicles (Evs). Recently, EVs are identified as a new mechanism in cell-to-cell communication by transfer of protein and genic information (mRNA, microRNA). Their role is under investigation in immunology, stem cell and cancer, but not in islets and diabetes. The aim of this experiment is to identify mRNA transcripts (in particular, mRNA transcripts involved in diabetes pathophysiology) present in islet Evs.

2012-08-02 | E-MEXP-3695 | biostudies-arrayexpress

SScHuEx

Project description:To determine the gene expression profile of endothelial cells derived from endothelial progenitor (EPCs) issued from EPC-derived endothelial cells collected from 10 SSc patients (5 patients with the diffuse cutaneous subset and 5 patient with the limited cutaneous subset) and 5 healthy controls before and after hypoxic exposure.

2012-01-20 | E-MEXP-3075 | biostudies-arrayexpress

microRNA expression in microvesicles (MVs) derived from endothelial progenitor cells (EPCs)

Project description:Evaluation of microRNA expression profile of microvesicles (MVs) derived from endothelial progenitor cells (EPCs) cultured in different oxygen concentrations (normoxic/hypoxic conditions)

2010-12-30 | E-MEXP-2956 | biostudies-arrayexpress

Transcription profiling of human immortalized pancreatic islet endothelial cells incubated with EPC-derived microvesicles

Project description:Endothelial cells derived from freshly purified human islets were incubated with microvesicles collected by ultracentrifugation of supernatants of endothelial progenitor cells (EPCs) isolated from peripheral blood of healthy volunteers.

2010-01-12 | E-MEXP-2511 | biostudies-arrayexpress

Deep RNA Sequencing of native mouse podocytes and non-podocytes glomerular cells

Project description:Purpose: Next-generation sequencing (NGS) was used to define the transcriptome of native mouse podocytes and non-podocytes glomerular cells as part of a project aiming to define the molecular fingerprint of mouse podocytes. Method: Glomeruli from 29 Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J x hNPHS2Cre mice at the age of 10 weeks were purified and a single cell solution was prepared to seperate GFP-expressing (podocytes) and GFP-negative (non-podocytes glomerular cells) cells by FACS sorting. RNA was extracted and prepared for further analysis using directional, polyA+ library preparation. An Illumina HiSeq2500 was used for a paired-end sequencing of 100 cycles . Salmon and Sleuth were used for downstream analysis. Results: A total of 100 Million reads each from podocytes and non-podocytes glomerular cells could be used for further analysis.

2019-01-01 | E-MTAB-5457 | biostudies-arrayexpress

RNA expression data from glomeruli lacking von Hippel-Lindau protein in podocytes

Project description:We and others have previously shown that glomerular endothelial cells and podocytes express hypoxia-inducible transcription factors (HIFs). HIFs bind to hypoxia response elements in target genes, such as vascular endothelial growth factor, which is continually produced by podocytes throughout life. To further assess function of HIFs in podocyte biology, podocin-Cre mice were mated with floxed von Hippel-Lindau (VHL) mice to selectively delete VHL, a component of an E3 ligase complex responsible for degradation of HIFs in normoxia. We reasoned that cells lacking VHL would overexpress stable HIFs and upregulate hypoxia-responsive genes. Progeny from these crosses displayed two phenotypes, non-proteinuric with glomerular basement membrane (GBM) defects and proteinuric with GBM defects and end-stage renal failure at ~6 months of age. Gene changes associated with the mild, non-proteinuric phenotype were studied using isolated glomeruli from wildtype and Pod-Cre fVHL mice. At 4 weeks of age, urine was collected and urinary albumin was quantified by Albuwell elisa from Pod-Cre fVHL litters. At 6 weeks of age, glomeruli from 3 wildtype littermate controls and 3 non-proteinuric Pod-Cre fVHL mice were collected using the magnetic bead method. RNA was extracted and hybridized to Affymetrix microarrays.

2010-08-03 | E-GEOD-20235 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data