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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of wild type Halobacterium salinarum R1 growing with or without aromatic amino acids

ABSTRACT: Medium-adapted cultures of H. salinarum were grown in synthetic medium with or without AroAA, and cells harvested by centrifugation after growth had reached OD600nm=0.2 and 0.58, respectively.

ORGANISM(S): Halobacterium salinarum

SUBMITTER: Mirit Kolog Gulko

PROVIDER: E-MEXP-3818 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

How do haloarchaea synthesize aromatic amino acids?

Gulko Miriam Kolog MK Dyall-Smith Mike M Gonzalez Orland O Oesterhelt Dieter D

PloS one 20140912 9

Genomic analysis of H. salinarum indicated that the de novo pathway for aromatic amino acid (AroAA) biosynthesis does not follow the classical pathway but begins from non-classical precursors, as is the case for M. jannaschii. The first two steps in the pathway were predicted to be carried out by genes OE1472F and OE1475F, while the 3rd step follows the canonical pathway involving gene OE1477R. The functions of these genes and their products were tested by biochemical and genetic methods. In thi ...[more]

PMID: 25216252

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