The interaction between glucose and cytokinin signal transduction pathway in Arabidopsis thaliana
Ontology highlight
ABSTRACT: Columbia (Col) seeds were sown on half-strength Murashige and Skoog (MS) medium, supplemented with 1% sucrose and 0.8% agar and grown vertically in culture room conditions. The 5-d-old homogenous seedlings were washed five times with sterile water and lastly with liquid half strength MS medium without sugar to remove residual exogenous sugar. In order to deplete internal sugars seedlings were grown in sugar free liquid half strength MS medium for 24 h in dark. Thereafter, the seedlings were treated with half-strength MS medium containing 0% G, 0% G + 1 uM BAP, 3% G, and 3% G + 1 uM BAP for 3 h in dark. RNA was extracted and microarray analysis was performed. Please note: G stands for glucose and BAP stands for 6-Benzylaminopurine (cytokinin)
ORGANISM(S): Arabidopsis thaliana
SUBMITTER: Ashverya Laxmi
PROVIDER: E-MEXP-3827 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
ACCESS DATA