Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of barley leaves after boron reveals a late response to boron toxicity

ABSTRACT: Barley1 GeneChip was used to investigate transcriptome changes associated with boron (B) toxicity in a sensitive barley cultivar (Hordeum vulgare L. cv. Hamidiye). Eight day old aseptically grown seedlings were subjected to 5 or 10 mM boric acid (B(OH)3) treatments for 5 days and expression profiles were determined with DNA microarrays using total RNA from leaf tissues. Surface sterilized seeds were aseptically germinated and grown on half strength Hoaglandâs solution (Hoagland and Arnon, 1950) (pH 5.8) solidified with PhytagelÂ® for 8 days at 23Â±2ÂºC with 16 h light (400 Âµmol mâ2 sâ1) and 8 h dark photo-cycle with 70% relative humidity. Seedlings were transferred to sterile hydroponic cultures for B treatment, which was applied immediately after transfer as half strength Hoaglandâs solution containing either 5 mM B(OH)3 (5B) or 10 mM B(OH)3 (10B) for another 5 days under the same physical conditions. Control (C) groups were transferred to half strength Hoaglandâs solution without extra B(OH)3. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Mehmet Tufan OZ. The equivalent experiment is BB63 at PLEXdb.] Experiment Overall Design: treated or untreated: Control (C)(3-replications); treated or untreated: 5 mM B(OH)3 treatment (5B)(3-replications); treated or untreated: 10 mM B(OH)3 treatment (10B)(3-replications)

ORGANISM(S): Hordeum vulgare

SUBMITTER: PLEXdb Curator Sudhansu Dash

PROVIDER: E-GEOD-14521 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Smoke released from burning vegetation functions as an important environmental signal promoting the germination of many plant species following a fire . It not only promotes the germination of species from fire-prone habitats, but several species from non-fire-prone areas also respond, including some crops. Bioactivity-guided fractionation of smoke-water led to the identification of a highly active butenolide compound, 3-methyl-2H-furo[2,3-c]pyran-2-one. Several hypotheses have arisen regarding the molecular background of smoke and butenolide action. Contrary to the efforts to unravel the mode of action of smoke, the mechanism is still largely unknown. In this paper we demonstrate that although smoke-water and butenolide treatment of maize kernels results in a similar physiological response, the gene and protein expression patterns are quite different. Treatment with smoke-water enhanced the ubiquitination of proteins and activated protein-degradation-related genes. This effect was completely absent from butenolide-treated kernels, in which a specific aquaporin gene was distinctly upregulated. These findings indicate that other bioactive compounds present in smoke-water may act together, leading to accelerated protein turnover. The results highlight the importance of protein degradation and aquaporins in the seed germination process. Besides their obvious use in the sustainable agricultural practice, smoke and butenolide can be used in studies to gain further insight into the transcriptional changes during germination. 15 individual kernels from each of six biological replicates were chosen and equal amount of the aaRNA samples were pooled. Three technical replicates were applied at each time point for the microarray analysis. For the control versus butenolide experiment the samples were collected at 1.5, 3, 6, 9, 12 and 24 hours after treatment. For the control versus smoke experiment the samples were collected at 1.5, 3, 6, 9, 12, 24 and 27 hours after treatment. For the delayed experiment samples were collected after 3 or 6 hours after treatment.

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Transcription profiling of barley leaves after boron reveals a late response to boron toxicity

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