Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

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Usyd_Wade_canFam2_60K_highDensity


ABSTRACT: A custom high density tiling path was designed to a 1.5Mb region of the canFam2.0 reference genome with probes tiled at 6bp or 26bp offsets. 60mer oligonucleotides were synthesized in situ to one 8 x 60K Agilent microarray and hybridized with canine genomic DNA. A single-channel hybridization protocol was employed using eight canine samples.

ORGANISM(S): Canis familiaris

SUBMITTER: Cali Willet 

PROVIDER: E-MEXP-3860 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Empirical assessment of competitive hybridization and noise in ultra high density canine tiling arrays.

Willet Cali E CE   Bunbury-Cruickshank Laura L   van Rooy Diane D   Child Georgina G   Shariflou Mohammad R MR   Thomson Peter C PC   Wade Claire M CM  

BMC bioinformatics 20130722


<h4>Background</h4>In addition to probe sequence characteristics, noise in hybridization array data is thought to be influenced by competitive hybridization between probes tiled at high densities. Empirical evaluation of competitive hybridization and an estimation of what other non-sequence related features might affect noisy data is currently lacking.<h4>Results</h4>A high density array was designed to a 1.5 megabase region of the canine genome to explore the potential for probe competition to  ...[more]

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