Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

49 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of Ercc1 white adipose tissue

ABSTRACT: Transcriptome analysis of epididymal white adipose tissue (WAT) depots in Ercc1 animals: To further elucidate the role of ERCC1 in WAT we scanned the transcriptome of 15 day old wt and Ercc1 epididymal WAT.

ORGANISM(S): Mus musculus

SUBMITTER: George Garinis

PROVIDER: E-MEXP-3930 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

DNA damage triggers a chronic autoinflammatory response, leading to fat depletion in NER progeria.

Karakasilioti Ismene I Kamileri Irene I Chatzinikolaou Georgia G Kosteas Theodoros T Vergadi Eleni E Robinson Andria Rasile AR Tsamardinos Iannis I Rozgaja Tania A TA Siakouli Sandra S Tsatsanis Christos C Niedernhofer Laura J LJ Garinis George A GA

Cell metabolism 20130901 3

Lipodystrophies represent a group of heterogeneous disorders characterized by loss of fat tissue. However, the underlying mechanisms remain poorly understood. Using mice carrying an ERCC1-XPF DNA repair defect systematically or in adipocytes, we show that DNA damage signaling triggers a chronic autoinflammatory response leading to fat depletion. Ercc1-/- and aP2-Ercc1F/- fat depots show extensive gene expression similarities to lipodystrophic Pparγ(ldi/+) animals, focal areas of ruptured basemen ...[more]

PMID: 24011075

Similar Datasets

MicroRNA profiling of mouse white adipose tissue, brown adipose tissue and skeletal muscle

Project description:Compare miRNA expression profiles in epididymal white adipose tissue (WAT), interscapular brown adipose tissue (BAT) and skeletal muscle from wild-type C57BL/6J mice

2010-12-01 | E-MEXP-2553 | biostudies-arrayexpress

Nck2 Regulates Adiposity and Adiposity-Related Metabolic Disorders in Mice and Human

Project description:Obesity is linked to the development of metabolic disorders. Expansion of white adipose tissue (WAT) from hypertrophy of pre-existing adipocytes and/or differentiation of precursors into new mature adipocytes contributes to obesity. We found that Nck2 expression is largely restricted to WAT, raising the hypothesis that it may play a unique function in that tissue. Using mice lacking Nck2, we found that Nck2 regulates adipocyte hypertrophy thus contributing to increased adiposity and progressive glucose intolerance, insulin resistance and hepatic steatosis. These findings were recapitulated in humans such that Nck2 expression in omental WAT was inversely correlated with the degree of obesity. Mechanistically, Nck2 deficiency promoted the induction of an adipocyte differentiation program and signaling by the PERK-eIF2Î±-ATF4 pathway in agreement with a role for the unfolded protein response in adipogenesis. These findings uncover Nck2 as a novel regulator of adipogenesis and that perturbation in its functionality contributes to adiposity-related metabolic disorders. Differential gene expression profile between epididymal white adipose tissue of Nck2-/- and Nck2+/+ mice by RNA sequencing (Illumina HiSEq 2000)

2016-07-01 | E-GEOD-63510 | biostudies-arrayexpress

Gene expression profiling of Ercc1-/- and age-matched wt pancreata

Project description:Total RNA was isolated from wt and Ercc1-/- pancreata from 15 days old C57BL/6J wt and Ercc1-/- mice

2021-05-15 | E-MTAB-10393 | biostudies-arrayexpress

MicroRNA profiling of mouse epididymal adipocytes from normal and leptin-deficient obese mice

Project description:Compare miRNA expression profiles in epididymal adipocytes from normal and leptin deficient obese mice

2009-02-21 | E-MEXP-1789 | biostudies-arrayexpress

MicroRNA profiling of mouse epididymal adipocytes from control and diet-induced obese mice

Project description:Compare miRNA expression profiles in epididymal adipocytes from control and diet-induced obese (DIO) mice

2009-02-25 | E-MEXP-1932 | biostudies-arrayexpress

Mouse expression data of epididymal white adipose tissue at Zeitgeber time (ZT) 18 comparison control vs. Sleep restriction

Project description:We hybridzed cRNA from epididymal white adipose tissue collected at ZT18 of control animals and TSR animals (TSR: these mice were sleep restricted for 6 hours every day by gentle handling for 5 consecutive days and killed on the last day at ZT18) mice used in this study were C57BL/6 control mice were compared to timed sleep restriction mice (TSR: these mice were sleep restricted for 6 hours every day by gentle handling for 5 consecutive days and killed on the last day at ZT18)

2015-06-15 | E-GEOD-38921 | biostudies-arrayexpress

Effect of dietary vitamin B6 on white adipose tissue

Project description:Transcriptional profiling of mouse white adipose tissues The objective of this study is to explore the relationship between vitamin B6 activity and chronic inflammation of white adipose tissue in mice fed a high-fat diet. Twenty-four CD-1 mice were divided into two groups (n = 12) and fed either a 1 mg pyridoxine (PN) HCl /kg diet or a 35 mg PN HCl /kg diet for 8 weeks. We isolated total RNA from epididymal white adipose tissue of each group and compared gene expression profiles by DNA microarray data analysis. Noriyuki Yanaka

2013-01-12 | E-GEOD-43465 | biostudies-arrayexpress

Proteomic analysis of adipose depots after intermittent fasting reveals visceral fat preservation mechanisms

Project description:Objectives Intermittent fasting is an effective dietary intervention to combat metabolic disease. Here, we explore the adipose depot specific response to every-other-day fasting (EODF) in mice to identify mechanisms that underly the beneficial effects. Methods Male C57BL/6J mice were placed on a 12-day EODF or ad libitum diet, after which tissues were harvested including visceral (vWAT) and subcutaneous (scWAT) white adipose tissue, as well as brown adipose tissue (BAT), which was then analysed by unbiased mass spectrometry-based proteomics. Results After EODF treatment, pathway enrichment analysis of our dataset showed that both WAT depots showed increased mitochondrial protein content, with scWAT also showing increased UCP1, but mitochondrial protein content was decreased in BAT. This effect on mitochondria is correlated to the increased abundance of proteins involved in glycolysis, pyruvate metabolism, the TCA cycle and fatty acid synthesis in both WAT depots. Furthermore, EODF-treated mice downregulated the lipolysis pathway in vWAT including a 5-fold decrease in the abundance of the beta3 adrenergic receptor (ADRB3). Enrichment analysis lso revealed that vWAT of EODF treated mice had significantly reduced ECM proteins, which lowers the inflammatory potential of this organ. Our adipose depot proteomic survey also allowed us to identify depot-enriched protein expression, such as the vWAT enrichment for the AKAP12 protein related to PKA signalling that was down-regulated by EODF treatment. Conclusions These findings show how the adipose depots have adapted to the EODF regime to preserve the lipid store, with the most striking changes occurring in the vWAT depot to downregulate the lipolysis pathway and induce expression of pathways needed for fatty acid synthesis. This substrate cycling and reduced inflammatory potential of the adipose tissue may contribute to the improved insulin sensitivity observed in these animals.

2022-10-12 | PXD020255 | Pride

Bulk RNA-seq of fibroblasts and mesothelial cells from healthy mouse tissues

Project description:Bulk RNA-seq was performed on fibroblasts and mesothelial cells from 11 different healthy mouse tissues such as bone, epididymal, brown and inguinal fat pads, mesentery, omentum, pancreas, liver, lung, lymph node and spleen. This data was used to examine tissue specific gene expression programs of fibroblasts and mesothelial cells.

2021-05-04 | E-MTAB-10324 | biostudies-arrayexpress

The effect of silibinin on protein expression profile in white adipose tissue of obese rats

Project description:According to different feeding and treatment conditions, 36 C57BL/6JC rats were randomly divided into normal diet group (WC group), high fat diet group (WF group) and high fat diet + silibinin group (WS group). TMT combined with LC-MS/MS were used to study the expression of WAT in epididymis of HFD-induced obese rats and normal diet rats. Gene Ontology, InterPro and KEGG databases were used to analyze the cellular processes, the biological processes, the corresponding molecular functions and the network molecular mechanisms involved

2020-01-13 | PXD016635 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data