Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Schmid_flowering_mutants_2013

ABSTRACT: Synchronized induction of flowering in Arabidopsis thaliana wild type (Col-0) and flowering time mutants (soc1, agl24, fd) by shifting from short day (8 hr light, 16 hr dark; 23C; 65% rel humidity) to long day (16 hr light, 8 hr dark; 23C; 65% rel humidity) for 0, 3, 5, and 7 days. Biotinylated probes were synthesized from RNA isolated from manually disseted shoot meristems and hybridized to Affymetrix ATH1 arrays.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Markus Schmid

PROVIDER: E-MEXP-4001 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

A quantitative and dynamic model of the Arabidopsis flowering time gene regulatory network.

Leal Valentim Felipe F Mourik Simon van Sv Posé David D Kim Min C MC Schmid Markus M van Ham Roeland C H J RC Busscher Marco M Sanchez-Perez Gabino F GF Molenaar Jaap J Angenent Gerco C GC Immink Richard G H RG van Dijk Aalt D J AD

PloS one 20150226 2

Various environmental signals integrate into a network of floral regulatory genes leading to the final decision on when to flower. Although a wealth of qualitative knowledge is available on how flowering time genes regulate each other, only a few studies incorporated this knowledge into predictive models. Such models are invaluable as they enable to investigate how various types of inputs are combined to give a quantitative readout. To investigate the effect of gene expression disturbances on fl ...[more]

PMID: 25719734

Similar Datasets

tps1-2_apices

Project description:To monitor gene expression changes pre floral transition and during early flower development col-0 and tps1-2 GVG::TPS1 (von Dijken, 2004) plants were grown under SD (8 hr light, 16 hr dark) for 21 days. Plants were then shifted to LD (16 hr light, 8 hr dark) conditions to induce flowering. RNA was isolated from micro-dissected apical tissue harvested 0 and 5 days after the shift to LD and double-stranded cDNA was synthesized. Biotinylated cRNA probes were prepared and hybridized to the Affymetrix ATH1 array in duplicate (biological replicates).

2013-02-15 | E-MEXP-3727 | biostudies-arrayexpress

Transcription profiling of Arabidopsis apices from control and smz-D flc-3 knock-out plants after photoperiod induction

Project description:Response of control (flc-3) and dominant late flowering mutant (smz-D flc-3) to inductive photoperiod (tissue: apices)

2009-06-05 | E-MEXP-2041 | biostudies-arrayexpress

Transcription profiling of Arabidopsis leaves from control and smz-D flc-3 knock-out plants after photoperiod induction

Project description:Response of Arabidopsis control (flc-3) and a dominant repressor of flowering (smz-D flc-3) to inductive photoperiod

2009-06-05 | E-MEXP-2040 | biostudies-arrayexpress

Transcription profiling of Arabidopsis thaliana LFY mutant plants transformed with different LFY orthologs to investigate evolutionary divergence of LFY function

Project description:The aim of this experiment is to test the ability of the ortholog of Arabidopsis LFY gene from flowering and non flowering species to complement an Arabidopsis LFY mutant. Plants expressing, in a homozygous lfy12 background, the open reading frame of LFY orthologs under the control of Arabidopsis LFY promoter were synchronously induced to flower by growing plants in short days for 30 days then shifting them to Long Day for an additional 8 days. Shoot apices were dissected at either d0 or d8 in long days. Two biological replicates were performed. The following genotypes were used: Col - wild type arabidopsis; reference strain LFY (lfy12; LFY::AthLFY) - Arabidopsis UNI (lfy12; LFY::UNI) - Pisum ALF (lfy12; LFY::ALF) - Petunia WelNDLY (lfy12; LFY::WelNDLY) - Welwitschia mirabilis CrLFY1 (lfy12; LFY::CrLFY1) - Ceratopteris richardii CrLFY2 (lfy12; LFY::CrLFY2) - Ceratopteris richardii lfy-2; weak lfy allele lfy-9; intermediate leafy allele lfy-12; strong leafy allele.

2009-07-23 | E-MEXP-2258 | biostudies-arrayexpress

circadian clock in cartilage

Project description:This experiment investigated the circadian clock in mice cartilage. A microarray time-series was performed using xiphoid tissue (metasternum) from mice in constant darkness. Time points were every 4 hr for 40 hr in total.

2013-10-19 | E-MEXP-3780 | biostudies-arrayexpress

Transcription profiling of potent induction of flowering in A. thaliana by elevated temperature

Project description:Plants were grown on soil for 35 days under short day conditions (8hrs light/16hrs dark) at 16¡ÄC and 65% relative humidity. Flowering was induced by shifting either to long day conditions (16/8) at 16¡ÄC and 65% relative humidity or to 25¨¬C in short days (8/16). Samples were taken at day 0, 2, 5 and 9 after shifting.

2006-06-02 | E-MEXP-728 | biostudies-arrayexpress

Transcription profiling of Arabidopsis thaliana AP1::AtIPT4 transformants vs wild type plants to identify the candidate genes responsive to cytokinin

Project description:The aim of this experiment is to identify the candidate genes responsive to cytokinin in Arabidopsis floral development. For this purpose, AtIPT4 gene under the control of the AP1 promoter was transformed into wild type plants of Arabidopsis. The transcriptional profiles of the young inflorescence of AP1::AtIPT4 transformants and wild-type plants are compared.

2010-09-07 | E-MEXP-2350 | biostudies-arrayexpress

Diamond Back Moth treatment

Project description:Arabidopsis thaliana plants (ecotype Landsberg erecta) were grown at 20˚C constant temperature, 8 hr/16 hr Light/Dark photoperiod at 50-60% ambient humidity, for 8 to 9 weeks. Short day conditions prevented the onset of flowering and the plants were thus maintained in growth stage 1 (leaf production) with 13 to 15 rosette leaves larger than 1mm (stage 1.13 to 1.14). Diamondback moth (DBM, Plutella xylostella) larvae were maintained on cabbage (Brassica oleracea) plants in a climate-controlled room at 25ºC, 12 hr photoperiod with 50%-60% relative humidity. Two days before exposing A. thaliana plants to herbivore treatment, plants were transferred to a climate-controlled room (22ºC, 50-60% humidity, 12 hr photoperiod). For insect treatment, seven Diamondback moth (DBM, Plutella xylostella) larvae (third to fifth instars) were placed on a group of four or five plants until time of harvest, for each time point separately. All rosette leaves were harvested at 1h, 4h, 12h, and 24h after onset of continuous herbivory and flash frozen in liquid nitrogen. Control plants were maintained under the same conditions and harvested in parallel. Keywords: time course, stress response, herbivory response

2008-03-04 | GSE10681 | GEO

Transcription profiling by array of Brachypodium response to increased ambient temperature in seedlings

Project description:Microarray analysis on Brachypodium distachyon seedlings was performed to determine the response of the transcriptome to changes in ambient temperature, including identification of marker genes that were up-regulated or down-regulated by a moderate increase in growth temperature. Wild-type Brachypodium (Bd21) seedlings were grown on MSR63 media without sucrose (Alves et al. 2009 Nature Protocols vol. 4 pp 638-649) in a short-day photoperiod (14 hr light/ 10 hr dark) at 17 ºC. As the third leaf was emerging, plants were transferred to 12 ºC for 48 hrs. Plants were then maintained at 12 ºC or transferred to one of two temperature treatments: constant 22 ºC or constant 27 ºC. Samples were collected before the shift (0 hr) and at 2 hr and 24 hr after the shift and immediately frozen in liquid nitrogen. For each harvest, two to three replicates were collected that each contained 3 seedlings.

2013-06-07 | E-MEXP-3918 | biostudies-arrayexpress

grf7-1

Project description:Arabidopsis thaliana (L.) Heynh. ecotype Columbia was used in this study. Seeds were sterilized and stratified at 4 C for 2 days in the dark, sown, and grown on germination media (GM) agar plates in a growth chamber with 16 hours of fluorescent light at 40M-110 ?mol m-2 sec-1, 22 C, and 70% relative humidity. The T-DNA insertion line for GRF7 was obtained from the ABRC (Stock No. CS878963;? Sessions et al., 2002). The T-DNA insertion was confirmed by amplifying the LB-flanking region in genomic DNA of individual candidate lines.

2013-02-12 | E-MEXP-3559 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data