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Transcription profiling of Arabidopsis thaliana LFY mutant plants transformed with different LFY orthologs to investigate evolutionary divergence of LFY function

ABSTRACT: The aim of this experiment is to test the ability of the ortholog of Arabidopsis LFY gene from flowering and non flowering species to complement an Arabidopsis LFY mutant.
Plants expressing, in a homozygous lfy12 background, the open reading frame of LFY orthologs under the control of Arabidopsis LFY promoter were synchronously induced to flower by growing plants in short days for 30 days then shifting them to Long Day for an additional 8 days. Shoot apices were dissected at either d0 or d8 in long days. Two biological replicates were performed. The following genotypes were used:
Col - wild type arabidopsis; reference strain
LFY (lfy12; LFY::AthLFY) - Arabidopsis
UNI (lfy12; LFY::UNI) - Pisum
ALF (lfy12; LFY::ALF) - Petunia
WelNDLY (lfy12; LFY::WelNDLY) - Welwitschia mirabilis
CrLFY1 (lfy12; LFY::CrLFY1) - Ceratopteris richardii
CrLFY2 (lfy12; LFY::CrLFY2) - Ceratopteris richardii
lfy-2; weak lfy allele
lfy-9; intermediate leafy allele
lfy-12; strong leafy allele.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: alexis Maizel

PROVIDER: E-MEXP-2258 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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