Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of brain in three adult humans and three adult chimpanzees

ABSTRACT: Gene expression profiling in brain of three adult humans and three adult chimpanzees

ORGANISM(S): Pan troglodytes

SUBMITTER: J.-E. Cheong

PROVIDER: E-MEXP-43 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

DNA sequence and comparative analysis of chimpanzee chromosome 22.

Watanabe H H Fujiyama A A Hattori M M Taylor T D TD Toyoda A A Kuroki Y Y Noguchi H H BenKahla A A Lehrach H H Sudbrak R R Kube M M Taenzer S S Galgoczy P P Platzer M M Scharfe M M Nordsiek G G Blöcker H H Hellmann I I Khaitovich P P Pääbo S S Reinhardt R R Zheng H-J HJ Zhang X-L XL Zhu G-F GF Wang B-F BF Fu G G Ren S-X SX Zhao G-P GP Chen Z Z Lee Y-S YS Cheong J-E JE Choi S-H SH Wu K-M KM Liu T-T TT Hsiao K-J KJ Tsai S-F SF Kim C-G CG OOta S S Kitano T T Kohara Y Y Saitou N N Park H-S HS Wang S-Y SY Yaspo M-L ML Sakaki Y Y

Nature 20040501 6990

Human-chimpanzee comparative genome research is essential for narrowing down genetic changes involved in the acquisition of unique human features, such as highly developed cognitive functions, bipedalism or the use of complex language. Here, we report the high-quality DNA sequence of 33.3 megabases of chimpanzee chromosome 22. By comparing the whole sequence with the human counterpart, chromosome 21, we found that 1.44% of the chromosome consists of single-base substitutions in addition to nearl ...[more]

PMID: 15164055

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Project description:Hepatitis A virus (HAV) is a hepatotropic human picornavirus that has been associated only with acute infection. Its pathogenesis is not well understood since there have been few recent studies in animal models using modern methodologies. We characterized HAV infections in three chimpanzees, quantifying viral RNA by qRT-PCR and examining critical aspects of the innate immune response including intrahepatic interferon-stimulated gene expression. We compared these infection profiles with similar studies of chimpanzees infected with hepatitis C virus (HCV), a hepatotropic flavivirus that frequently causes persistent infection. Surprisingly, HAV-infected animals exhibited very limited induction of type I interferon-stimulated genes in the liver compared to chimpanzees with acute resolving HCV infection, despite similar levels of viremia and 100-fold greater quantities of viral RNA in the liver. Minimal ISG15 and IFIT1 responses peaked 1-2 weeks after HAV challenge, then subsided despite continuing high hepatic viral loads. An acute inflammatory response at 3-4 weeks correlated with the appearance of virus-specific antibodies, and both apoptosis and proliferation of hepatocytes. Despite this, HAV RNA persisted in the liver for months, remaining present long after its clearance from serum and feces and revealing dramatic differences in the kinetics of clearance in the three compartments. Viral RNA was detected in the liver for significantly longer (35 to >48 weeks) than HCV RNA in animals with acute resolving HCV infection (10-20 weeks). Collectively, these findings suggest that early HAV infection is far stealthier than HCV infection and represents a distinctly different paradigm in viral-host interactions within the liver. Chimpanzee liver was biopsied during an acute HAV infection. Chimp 1 and 2 had two baseline samples. Chimp 3 used the baselines from chimps 1 and 2. Chimp 1 had 8 samples during the HAV acute infection. Chimp 2 had 9 samples during the HAV acute infection. Chimp 3 had 4 samples during the HAV acute infection.

Dataset Information

Transcription profiling of brain in three adult humans and three adult chimpanzees

Publications

DNA sequence and comparative analysis of chimpanzee chromosome 22.

Similar Datasets

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