Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of individual resistant and infected epidermalsingle cells of the susceptible barley line Pallas

ABSTRACT: Investigation of the interaction between barley and B. graminis. Individual resistant and infected epidermal cells of the susceptible barley line Pallas were easily distinguished by microscopy, and their contents collected in micro-capillaries. We hybridized 12 arrays with radioactively labeled material from these cell specific samples; 3 with material from resistant cells, 3 with material from infected cells, 3 with material from non-inoculated control cells, and 3 identical samples for technical replication. Pooling equal amounts of material from the 3 infected samples made these last 3 samples.

INSTRUMENT(S): Fujix BAS 1000

ORGANISM(S): Hordeum vulgare

DISEASE(S): resistant

SUBMITTER: Peter Hagedorn

PROVIDER: E-MEXP-457 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Single-cell transcript profiling of barley attacked by the powdery mildew fungus.

Gjetting Torben T Hagedorn Peter H PH Schweizer Patrick P Thordal-Christensen Hans H Carver Timothy L W TL Lyngkjaer Michael F MF

Molecular plant-microbe interactions : MPMI 20070301 3

In many plant-pathogen interactions, there are several possible outcomes for simultaneous attacks on the same leaf. For instance, an attack by the powdery mildew fungus on one barley leaf epidermal cell may succeed in infection and formation of a functional haustorium, whereas a neighboring cell attacked at the same time may resist fungal penetration. To date, the mixed cellular responses seen even in susceptible host leaves have made it difficult to relate induced changes in gene expression to ...[more]

PMID: 17378426

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Dataset Information

Transcription profiling of individual resistant and infected epidermalsingle cells of the susceptible barley line Pallas

Publications

Single-cell transcript profiling of barley attacked by the powdery mildew fungus.

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