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Livers from lean or fat muscle lines fed low or high lipid diets


ABSTRACT: We used fish from two divergent lines of rainbow trout, selected for low or high muscle fat content (respectively L and F lines) and fed two different diets, containing either 10% (LE diet) or 23% (HE diet) lipids. For more details about experimental fish and diets, see Kolditz et al.,Am J Physiol Regul Integr Comp Physiol 294: R1154–R1164, 2008. There are 4 experimental conditions: L-LE, L-HE, F-LE and F-HE. This experiment contain the data derived from the hybridization of the RNA extracted from individual liver of these fish. for each membrane, spots with an oligonucleotide signal lower than three times the background level were excluded from the analysis. After this filtering step, signal processing was performed using the vector oligonucleotide data to correct each spot signal according to the actual amount of DNA present in each spot. After correction, the signal was normalized by dividing each gene expression value by the median value of the array. Keywords: Nutritional genomics, response to divergent selection for muscle fattening, liver metabolism, rainbow trout 32 samples

ORGANISM(S): Oncorhynchus mykiss

SUBMITTER: catherine kolditz 

PROVIDER: E-GEOD-12031 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Changes induced by dietary energy intake and divergent selection for muscle fat content in rainbow trout (Oncorhynchus mykiss), assessed by transcriptome and proteome analysis of the liver.

Kolditz Catherine-Ines CI   Paboeuf Gilles G   Borthaire Maïena M   Esquerré Diane D   SanCristobal Magali M   Lefèvre Florence F   Médale Françoise F  

BMC genomics 20081029


<h4>Background</h4>Growing interest is turned to fat storage levels and allocation within body compartments, due to their impact on human health and quality properties of farm animals. Energy intake and genetic background are major determinants of fattening in most animals, including humans. Previous studies have evidenced that fat deposition depends upon balance between various metabolic pathways. Using divergent selection, we obtained rainbow trout with differences in fat allocation between vi  ...[more]

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