Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

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Transcription profiling of Mus musculus, Bos taurus, Xenopus laevis oocytes


ABSTRACT: Identification of genes expressed in oocytes and conserved in three different species with a multi-species cDNA microarray.

INSTRUMENT(S): VersArray ChipReader [Bio-Rad Laboratories]

ORGANISM(S): Xenopus laevis

SUBMITTER: Maud Vallee 

PROVIDER: E-MEXP-488 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Identification of novel and known oocyte-specific genes using complementary DNA subtraction and microarray analysis in three different species.

Vallée Maud M   Gravel Catherine C   Palin Marie-France MF   Reghenas Hélène H   Stothard Paul P   Wishart David S DS   Sirard Marc-André MA  

Biology of reproduction 20050302 1


The main objective of the present study was to identify novel oocyte-specific genes in three different species: bovine, mouse, and Xenopus laevis. To achieve this goal, two powerful technologies were combined: a polymerase chain reaction (PCR)-based cDNA subtraction, and cDNA microarrays. Three subtractive libraries consisting of 3456 clones were established and enriched for oocyte-specific transcripts. Sequencing analysis of the positive insert-containing clones resulted in the following classi  ...[more]

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