Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human NIH 3T3 inducible cell line to study the role of PKR in regulating gene expression


ABSTRACT: PKR is an interferon induced serine/threonine protein kinase, that is activated by double stranded RNA. PKR plays an important role in the antiviral defense by interferon. In addition to its role in translation, PKR participates in several signaling pathways to transcription. The goal of this experiment is to study the role of PKR in regulating gene expression in our NIH 3T3 inducible cell line, which could overexpress PKR wt protein after the removal of tetracycline (Donze O, Dostie J, Sonenberg N. (1999) Virology 256: 322-9).

ORGANISM(S): Mus musculus

SUBMITTER: Rob Sladek 

PROVIDER: E-MEXP-50 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The protein kinase PKR: a molecular clock that sequentially activates survival and death programs.

Donzé Olivier O   Deng Jing J   Curran Joseph J   Sladek Robert R   Picard Didier D   Sonenberg Nahum N  

The EMBO journal 20040129 3


Cell death and survival play a key role in the immune system as well as during development. The control mechanisms that balance cell survival against cell death are not well understood. Here we report a novel strategy used by a single protein to regulate chronologically cell survival and death. The interferon-induced protein kinase PKR acts as a molecular clock by using catalysis-dependent and -independent activities to temporally induce cell survival prior to cell death. We show that the proapo  ...[more]

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