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Transcription profiling of mouse Nkx2-5 null cardiomyocytes

ABSTRACT: Nkx2-5-GFP+ cells were collected by FACS from whole embryos (E8.0-8.5, 5-12 ps) and dissected heart tubes (E9.0-9.5, 15-21 ps). Because limited numbers of cells were obtained from individual embryos (~300 cells at E8, ~1500 cells at E9.5) mRNA was amplified twice with T7 RNA polymerase and fluorescent cDNA targets were synthesized by the indirect method with either Cy5 or Cy3. Targets from individual Nkx2-5 heterozygous and homozygous null cells were co-hybridized with cDNA probes printed on glass slide microarrays. Two microarray clone sets were used for each hybridization: the NIA/NIH 15,000 clone set derived from mouse non-cardiac embryonic tissue, and a ~6000 cardiac clone set derived in house.

INSTRUMENT(S): GenePix 4000A [Axon Instruments]

ORGANISM(S): Mus musculus

SUBMITTER: Owen Prall

PROVIDER: E-MEXP-533 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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