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Transcription profiling of CACNA1E overexpression in human embryonic kidney HEK293 cells to investigate the involvement of CACNA1E in Wilms tumorigenesis

ABSTRACT: Wilms tumour (nephroblastoma) is the most common paediatric renal tumour, affecting 1 in 10,000 children. Despite the excellent survival of patients treated with a combination of surgery, chemotherapy and radiotherapy, approximately 15% will relapse and die of this disease. We have identified CACNA1E to be significantly overexpressed in relapsing tumours compared to non-relapsing. To investigate the involvement of CACNA1E in Wilms tumorigenesis, CACNA1E stably transfected HEK293 cell line and parental lines were obtained, grown and RNA extracted. To assess differential gene expression between the two cell lines, RNA was hybridised to Affymetrix Human Genome U133 Plus 2.0 GeneChip. Human embryonic kidney HEK293 parental and HEK2C6, (CACNA1E stably transfected cells) were kindly provided by Prof. Schneider, Cologne (Pereverzev et al., 1998).

ORGANISM(S): Homo sapiens

SUBMITTER: Gordan Vujanic 

PROVIDER: E-MEXP-587 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Amplification and overexpression of CACNA1E correlates with relapse in favorable histology Wilms' tumors.

Natrajan Rachael R   Little Suzanne E SE   Reis-Filho Jorge S JS   Hing Lara L   Messahel Boo B   Grundy Paul E PE   Dome Jeffrey S JS   Schneider Toni T   Vujanic Gordan M GM   Pritchard-Jones Kathy K   Jones Chris C  

Clinical cancer research : an official journal of the American Association for Cancer Research 20061201 24

<h4>Purpose</h4>The most well established molecular markers of poor outcome in Wilms' tumor are loss of heterozygosity at chromosomes 1p and/or 16q, although to date no specific genes at these loci have been identified. We have previously shown a link between genomic gain of chromosome 1q and tumor relapse and sought to further elucidate the role of genes on 1q in treatment failure.<h4>Experimental design</h4>Microarray-based comparative genomic hybridization identified a microamplification harb  ...[more]

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