Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Xenopus tropicalis wild type and whitehart embryos


ABSTRACT: Two batches each of 40 wild type and 40 whitehart sibling embryos were sorted by morphological criteria at stage 37-38, RNA prepped and hybed to arrays. With mutant versus wild type sibling pools. Dyeswaps were performed.

INSTRUMENT(S): ProScanArray Microarray Scanner [PerkinElmer]

ORGANISM(S): Xenopus tropicalis

DISEASE(S): whitehart

SUBMITTER: Lyle Zimmerman 

PROVIDER: E-MEXP-691 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genetic screens for mutations affecting development of Xenopus tropicalis.

Goda Tadahiro T   Abu-Daya Anita A   Carruthers Samantha S   Clark Matthew D MD   Stemple Derek L DL   Zimmerman Lyle B LB  

PLoS genetics 20060609 6


We present here the results of forward and reverse genetic screens for chemically-induced mutations in Xenopus tropicalis. In our forward genetic screen, we have uncovered 77 candidate phenotypes in diverse organogenesis and differentiation processes. Using a gynogenetic screen design, which minimizes time and husbandry space expenditures, we find that if a phenotype is detected in the gynogenetic F2 of a given F1 female twice, it is highly likely to be a heritable abnormality (29/29 cases). We  ...[more]

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