Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of upper beak primorida of five species of Darwins Finches to identify species and/or size specific transcripts

ABSTRACT: We used five different species of Darwins Finches (G. magnirostris, G. conirostris, G. fortis, G. scandens, G. difficilis). G. difficilis was used as a common reference and we polled RNA from 9 unrelated individuals of this species to lower variation. We used at least two unrelated individual embryos from each of the more derived species. We first made cDNA library from the upper beak primordia tissue, which was used to print microarrays. Next, we collected upper beak primordia from stage 26 embryos of 5 species of Darwins Finches that differed in their beak morphology, for making Cy3- and Cy5-labeled probes with the most basal species, the Sharp-beaked Finch, serving as a reference. We then clustered the obtained expression profiles to find transcripts that were expressed in a species- and/or size-specific manner.

INSTRUMENT(S): Axon- GenePix4000B

ORGANISM(S): Geospiza magnirostris

SUBMITTER: Clifford Tabin

PROVIDER: E-MEXP-702 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The calmodulin pathway and evolution of elongated beak morphology in Darwin's finches.

Abzhanov Arhat A Kuo Winston P WP Hartmann Christine C Grant B Rosemary BR Grant Peter R PR Tabin Clifford J CJ

Nature 20060801 7102

A classic textbook example of adaptive radiation under natural selection is the evolution of 14 closely related species of Darwin's finches (Fringillidae, Passeriformes), whose primary diversity lies in the size and shape of their beaks. Thus, ground finches have deep and wide beaks, cactus finches have long and pointed beaks (low depth and narrower width), and warbler finches have slender and pointed beaks, reflecting differences in their respective diets. Previous work has shown that even smal ...[more]

PMID: 16885984

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Dataset Information

Transcription profiling of upper beak primorida of five species of Darwins Finches to identify species and/or size specific transcripts

Publications

The calmodulin pathway and evolution of elongated beak morphology in Darwin's finches.

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