Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

45 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of wild type B. cereus and a deltion mutant of the PlcR regulator at two time points

ABSTRACT: Comparison at two different timepoints of expression profiles of wild_type Bacillus and a deletion mutant of the PlcR regulator. Additional processed data can be found in the FTP directory for this experiment ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/MEXP/E-MEXP-1472/

INSTRUMENT(S): GenePix 4000B [Axon Instruments]

ORGANISM(S): Bacillus cereus

SUBMITTER: Karoline Faegri

PROVIDER: E-MEXP-1472 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of Yersinia pestis overexpressing phoP

Project description:Microarrays were used to identify Y. pestis genes that are differentially regulated under conditions of phoP overexpression. RNA samples were isolated from cultures of two isogenic Y. pestis strains, one overexpressing phoP (KIM10+phoP(delta)/PhoP) and the other not (KIM10+phoP(delta)/MMB67EH), and their gene expression profiles were compared.

2006-07-01 | E-MEXP-712 | biostudies-arrayexpress

Transcription profiling of Yersinia pestis wild type and phoP deletion strains

Project description:Microarrays were used to identify Y. pestis genes that are transcriptional targets of phoP. RNA samples were isolated from cultures of two isogenic Y. pestis strains, one carrying wild-type phoP (KIM6+) and the other carrying a deletion within phoP (KIM6+phoP(delta)), and their gene expression profiles were compared.

2006-07-01 | E-MEXP-713 | biostudies-arrayexpress

Transcription profiling of human fibroblasts from foreskin to determine changes in miRNA regulation with age

Project description:Foreskin samples were derived from young and old donors. Total RNA was extracted and used for hybridization to microRNA microarrays. From 4 young and 4 old donors, differential expression of microRNAs was analyzed using R/Bioconductor and linear models

2010-04-19 | E-MEXP-2459 | biostudies-arrayexpress

Transcription profiling of human miRNA expression in mesenchymal stem cells in young and old donors.

Project description:Mesenchymal stem cells were isolated from several donors of different age (young vs old), and total RNA was extracted. Samples were labeled with Cy3 and Cy5 dyes and hybridized in a looped design that allowed the calculation of differentially expressed miRNAs in MSCs isolated from healthy, aged individuals.

2010-04-19 | E-MEXP-2455 | biostudies-arrayexpress

MicroRNA profiling of human umbical vein endothelial cells (HUVEC) using young (replicating) cells and senescent cells

Project description:From two donors of human umbilical vein endothelial cells, in vitro cell lines were established. Both cell lines were grown in vitro until irreversible growth arrest was observed (replicative senescence). Total RNA from young (replicating) cells as well as senescent cells was harvested and used for hybridization of microRNA chips (MRC) from TU Graz based on Sanger miRBase 9.2

2010-04-19 | E-MEXP-2425 | biostudies-arrayexpress

Transcription profiling of rat heart transplants from Lewis to Lewis and Lewis to F344 strains with and without cold storage / prolonged cold ischemia.

Project description: Annual heart allograft failure in humans rates about 3-5%. The main reason after the first postoperative year is chronic rejection. Myointimal hyperplasia, the hellmark of chronic rejection, results in a specific type of ischemic heart disease. The lack of angina pectoris symptoms allow ventricular arrythmias, sudden cardiac death or heart failure to occur without warning. In addition, diagnostic tools such as endomyocardial biopsy, coronary angiography or intracoronary ultrasound fail to predict the individual risk for myocardial dysfunction. The mechanisms responsible for chronic rejection are predominantly alloimmune mediated with activated T cells, macrophages, B cell mediated antibody formation and secreted cytokines responding to HLA and other endothelial cell antigens. In addition, non immunologic risk factors such as recipient age, metabolic factors, hypertension and ischemia contribute to development of this disease. Previous studies have demonstrated that ischemia has a profound influence on short term allograft survival but the underlaying mechanisms remain largely unknown. Apoptosis seems to play a crucial role in ischemia/reperfusion injury and several mechanisms for programmed cell death have been described. However, consequences on long term cell function of viability have not been investigated. The aim of this study was to investigate the implication and the mechanism of prolonged cold organ storage as a non immunologic risk factor in the pathogenesis of chronic rejection in a cardiac allograft model. We aimed for answering the following specific questions: How does cold ischemia affect the alloimmue response short and long term? How does prolonged cold ischemia affect gene expression at later time points after transplantation? Does it influence gene expression during chronic rejection?

2010-10-13 | E-MEXP-2301 | biostudies-arrayexpress

MicroRNA profiling of human CD8 T cells - miR-17 and miR-19b to investigate down-regulatation in human aging

Project description:T cells from several blood donors were separated in populations corresponding to CD8+ CD28+ and CD8+ CD28- surface markers. Total RNA extracts from cells were hybridized to MRC LNA microarray. Contrasts of interest were CD8CD28- minus CD8CD28+ (replicative aging) and CD8CD28+ (old donors) minus CD8CD28+ (young donors), which corresponds to chronological aging.

2010-04-19 | E-MEXP-2398 | biostudies-arrayexpress

Transcription profiling of Picea glauca xylem and phloem

Project description:In this experiment we compared the transcriptomes of xylem and phloem.

2009-03-04 | E-MEXP-1489 | biostudies-arrayexpress

Transcription profiling of mouse peripheral blood mononuclear cells and brain after maternal separation

Project description:We used a mouse maternal separation model, a well-known paradigm of early adversity, to test the hypothesis that transcriptional changes in peripheral blood mononuclear cells (PBMCs) are paralleled by specific gene expression changes in three brain regions that are involved in the stress response. Furthermore, we evaluated whether gene expression profiles of PBMCs could be used to predict stress-related animal behaviours.

2009-07-31 | E-MEXP-2101 | biostudies-arrayexpress

Antibody microarray profiling of pancreatic cancer using 12 urine samples

Project description:Antibody microarray based profiling of twelve urine samples. 3 healthy female 3 heatlhy male 3 female with pancreatic cancer 3 male with pancreatic cancer

2012-05-03 | E-MEXP-3006 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data