Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

53 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of hippocampus from wild type and CIC-6 knock-out mice

ABSTRACT: 3 pairs of wt and ClC-6 knockout mice, RNA from p14 hippocampus

ORGANISM(S): Mus musculus

SUBMITTER: Jens Fuhrmann

PROVIDER: E-MEXP-731 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Lysosomal storage disease upon disruption of the neuronal chloride transport protein ClC-6.

Poët Mallorie M Kornak Uwe U Schweizer Michaela M Zdebik Anselm A AA Scheel Olaf O Hoelter Sabine S Wurst Wolfgang W Schmitt Anja A Fuhrmann Jens C JC Planells-Cases Rosa R Mole Sara E SE Hübner Christian A CA Jentsch Thomas J TJ

Proceedings of the National Academy of Sciences of the United States of America 20060901 37

Mammalian CLC proteins function as Cl(-) channels or as electrogenic Cl(-)/H(+) exchangers and are present in the plasma membrane and intracellular vesicles. We now show that the ClC-6 protein is almost exclusively expressed in neurons of the central and peripheral nervous systems, with a particularly high expression in dorsal root ganglia. ClC-6 colocalized with markers for late endosomes in neuronal cell bodies. The disruption of ClC-6 in mice reduced their pain sensitivity and caused moderate ...[more]

PMID: 16950870

Similar Datasets

Transcription profiling of wild type and Clcn7-/- mouse hippocampus at p14

Project description:Comparison of expression levels between wild type and Clcn7-/- mouse hippocampus at p14. 3 wild type and 3 mutant mice were studied.

2005-03-01 | E-MEXP-242 | biostudies-arrayexpress

Transcription profiling of mouse KO Clcn5-/- model for Dents disease in kidney, bone and intestine

Project description:Mutations in ClC-5 cause Dent's disease, a disorder associated with low molecular weight proteinuria, hyperphosphaturia and kidney stones. ClC-5 is a Cl /H+-exchanger predominantly expressed in the kidney, where it facilitates the acidification of proximal tubular endosomes. The reduction in proximal tubular endocytosis resulting from a lack of ClC-5 raises the luminal concentration of filtered proteins and peptides like PTH.<br><br> We used gene expression profiling to identify possible signaling pathways that might be changed in ClC-5 KO kidneys, bones and intestines.<br%gt;<br> Mouse model described in Piwon et al, ClC-5 Cl--channel disruption impairs endocytosis in a mouse model for Dent's disease, Nature 408, 369-373 (16 November 2000),doi: 10.1038/35042597

2006-06-13 | E-MEXP-495 | biostudies-arrayexpress

Transcription profiling of chloride channel ClC-2 knock out mice with a Leukoencephalopathy-like phenotype vs.wild type

Project description:ClC-2 is a broadly expressed Cl- channel of the CLC family of Cl- channels and transporters which is abundantly expressed in brain. Here it was proposed to participate in lowering the cytoplasmic Cl- concentration of neurons, a process that establishes an inhibitory response to the neurotransmitters GABA and glycine (Staley et al., 1996). Heterozygous mutations in CLCN2 (the gene encoding ClC-2) were recently reported in a few patients with three clinically distinct forms of epilepsy (Haug et al, 2003). However, the disruption of ClC-2 in mice (ClC-2 KO mouse) did not entail epilepsy (BÃÂ¶sl et al., 2001; Nehrke et al., 2002) but myelin vacuolation in fiber tracts of the central nervous system. We used a gene expression profiling of the ClC-2 KO mouse in brain to identify possible disease mechanism which cause the observed myelin phenotype. As these myelin vacuolation became apparent in the fiber tracts of ClC-2 KO cerebellum at P28 and increased with age, we analysed the cerebellum of ClC-2 KO mice at different postnatal ages, before (P14) and after (P35) the KO cerebellum has been affected by myelin vacuolation.

2008-05-01 | E-MEXP-1028 | biostudies-arrayexpress

Transcription profiling of wild type dorsal root ganglia (DRG) taken from mice 10-12 weeks, DRG taken from NT-4 -/- mice of 4-5 weeks, NT-4 -/- mice of 12 weeks

Project description:Gene expression profiling was carried out to compare labeled cRNA derived from 3 experimental groups. Group 1 was mRNA extracted from wild type dorsal root ganglia (DRG) taken from mice 10-12 weeks of age, Group 2 was mRNA extracted from DRG taken from NT-4 -/- mice of 4-5 weeks of age. Group 3 was mRNA extracted from DRGs taken from NT-4 -/- mice of 12 weeks of age. In all cases mRNA was extracted from 6-10 mice per group. The experiment was carried out a total of three times.

2012-04-19 | E-MEXP-10 | biostudies-arrayexpress

Transcription profiling of 10-day old Arabidopsis seedlings treated for 30, 120 and 240 minutes with the plant hormone indole-3-acetic acid (auxin)Auxin induced effects investigated using bead-purified probe arrays to assess the technology

Project description:21120 Arabidopsis thaliana gene sequence tags were amplified and purified using a novel bead-based strategy (see Publication for more details).This experiment was carried out to demonstrate the use of these arrays for expression profiling of Arabidopsis thaliana.Briefly, 10-day old Arabidopsis seedlings were treated for 30, 120 and 240 minutes with the plant hormone indole-3-acetic acid (auxin) and the effects on gene expression were analysed.

2004-12-31 | E-MEXP-140 | biostudies-arrayexpress

Transcription profiling of indole-3-acetic acid at physiological concentrations (IAA) treatment of Arabidopsis seedlings

Project description:To demonstrate the analysis of differential expression using CATMA v1 arrays, Arabidopsis seedlings were treated with indole-3-acetic acid at physiological concentrations. The seedlings were germinated in liquid medium, and treated with indole-3-acetic acid for 30, 120 or 240 minutes. Changes in expression patterns were monitored using a complete loop design including an untreated sample (0 minutes). Reciprocal labelling was used rendering a total of eight hybridisations. An additional self-to-self hybridisation for time-point 0 was included. The statistical analysis was based an ANOVA model and indicated that 1123 GSTs were differentially expressed (p < 2.37 x 10-6) in at least one of the three time points following treatment.

2004-05-31 | E-MEXP-65 | biostudies-arrayexpress

Transcription profiling of pNCF (non-conjugative factor of F plasmid) carrying E.coli biofilm

Project description:Three E.coli affymetrix antisense arrays were used to examine the global gene expression of pNCF carrying E.coli biofilm. The pNCF is the derivative of only 65 kb non-conjugative factor of F plasmid. It was introduced to E.coli MG1655 strains before prepare the biofilm samples. The biofilms were cultured under the continuous flow cell system using MOP minimal medium supplemented with 0.02% glucose at 37C. The total RNA was extracted directly from the chamber after 48h of incubation for further array procedures according to the manufacture manual.

2007-09-01 | E-MEXP-1145 | biostudies-arrayexpress

Transcription profiling of E. coli biofilm to assess the global gene impact of a natural F conjugative plasmid-carrying E.coli biofilm

Project description:we examined the three different mature biofilms and searched the genes which promoted the rapid biofilm formation when their population hosing the plasmids. We investigate the global transcriptional differences between the non-conjugative or conjugative plasmid-carrying and plasmid-free strains.

2007-05-15 | E-MEXP-953 | biostudies-arrayexpress

Role of the Ccr4/Not complex in the regulation of heterochromatin in Schizosaccharomyces pombe (IV)

Project description:Expression profiling of mutants of genes encoding components of the Ccr4-Not complex and other related genes

2015-09-25 | E-MTAB-3059 | biostudies-arrayexpress

Role of the Ccr4/Not complex in the regulation of heterochromatin in Schizosaccharomyces pombe (V)

Project description:Expression profiling of mutants of genes encoding components of the Ccr4-Not complex and other related genes

2015-09-25 | E-MTAB-3060 | biostudies-arrayexpress