Transcription profiling of Arabidopsis plants after 2 hr or 10 hrs salt treatment
Ontology highlight
ABSTRACT: Gene expression was compared between non-stress Arabidopsis plants (0 hr salt treatment) and salt treated plants (2 hrs and 10 hrs salt treatment).
INSTRUMENT(S): DNA Microarray Scanner BA [Agilent Technologies]
Project description:ZmDREB2A is a DREB2-type transcription factor cloned from maize, whose transcript was upregulated by drought, high salt, low temperature and heat stresses. The ZmDREB2A gene possesses two kinds of transcription forms by alternative splicing. Only the functional form was studied to be highly induced by stresses. Transgenic plants overexpressing ZmDREB2A (35S:ZmDREB2A) showed dwarfism and enhanced drought stress tolerance. Microarray analysis of two independent transgenic plants revealed that in addition to genes encoding LEA proteins, some genes related to heat shock and detoxification were also upregulated. Experiments on termotolerance tests of these transgenic plants showed overexpressing ZmDREB2A gene also improved plant tolerance to heat stress.
Project description:DREB2A is a transcription factor responsive to drought, high salt and high temperature stress.In this experiment, gene expression profiles of DREB2A KO mutants were compared with wild type under stress condition.
Project description:ATHK1 is a histidine kinase responsive to drought stress.In this experiment, gene expression profile of athk1 knockdown mutant was compared with that of the wild_type under unstressed condition.
Project description:AREB1deltaQT is a constitutively active form of AREB1, an abscisic acid (ABA)-responsive element binding protein (Uno et al., 2000, PNAS 97: 11632-11637). AREB1 is a bZIP transcription factor in Arabidopsis plants (Col). transcription of the AREB1 gene is upregulated by drought, NaCl, and ABA treatment in vegetative tissues. Because expression of the intact AREB1 gene on its own is insufficient to lead to expression of downstream genes under normal growth conditions, we created an activated form of AREB1 (AREB1deltaQT) to overcome the masked transactivation activity of AREB1. In this experiment, to help to identify target genes of AREB1, we compared the expression profiles in two independent lines 35S-AREB1deltaQT plants under non-stress conditions with that of wild-type plants by using Agilent near-full-genome gene chips (Arabidopsis 22K).
Project description:Constitutive active form of DREB2A, a transcriptional regulator involving to plant drought and high-salinity stress response, was overexpressed in Arabidopsis, and gene expression pattern was compared between transgenic plants and wild type plants.
Project description:To identify genes acting downstream of MAP kinase kinase, and thus map its signal, we used the steroid-inducible overexpression system. The constitutive active form MKK3 (MKK3DD) or MKK4 (MKK4DD) was cloned into the glucocorticoid-inducible vector pTA7002 and transformed using Agrobacterium method into Arabidopsis Col-0 plants. The transgenic plants transformed empty vector was used as control plants (Cont).
Project description:Gene expressions were compared between non-stress Arabidopsis plants (0 hr at low-temperature treatment) and low-temperature treated plants (2 hrs and 10 hrs low-temperature treatment).