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Chromatin immunoprecipitation of Zea mays chloroplast extracts using antibodies raised against OE17 and OE23 chloroplast targeted proteins

ABSTRACT: OE17 and OE23 are chloroplast targeted proteins. RNA co-immunoprecipitation was performed using antibodies raised against these proteins. RNA from the pellet and from the supernatent for each pulldown was labeled with different fluoro-dyes.

INSTRUMENT(S): Axon GenePix 4000B scanning hardware

ORGANISM(S): Zea mays

SUBMITTER: Christian Schmitz-Linneweber

PROVIDER: E-MEXP-784 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A pentatricopeptide repeat protein facilitates the trans-splicing of the maize chloroplast rps12 pre-mRNA.

Schmitz-Linneweber Christian C Williams-Carrier Rosalind E RE Williams-Voelker Pascale M PM Kroeger Tiffany S TS Vichas Athea A Barkan Alice A

The Plant cell 20061013 10

The pentatricopeptide repeat (PPR) is a degenerate 35-amino acid repeat motif that is widely distributed among eukaryotes. Genetic, biochemical, and bioinformatic data suggest that many PPR proteins influence specific posttranscriptional steps in mitochondrial or chloroplast gene expression and that they may typically bind RNA. However, biological functions have been determined for only a few PPR proteins, and with few exceptions, substrate RNAs are unknown. To gain insight into the functions an ...[more]

PMID: 17041147

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