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RNAi knock down of VCP in human HeLa cells

ABSTRACT: Study of the effects of the VCP knockdown. VCP (p97, yeast cdc48) is a hexameric AAA ATPase involved in various cellular functions including degradation of proteins by the ubiquitin-proteasome system. We examine the consequences of the reduction of VCP levels after RNAi of VCP in HeLa cells. We find ~30 transcripts upregulated in a sequence independent manner. Those transcripts encode proteins involved in endoplasmic reticulum stress, apoptosis, and amino acid starvation.

INSTRUMENT(S): GenePix 4000A [Axon Instruments]

ORGANISM(S): Homo sapiens

SUBMITTER: Maga Rowicka

PROVIDER: E-MEXP-817 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Valosin-containing protein (p97) is a regulator of endoplasmic reticulum stress and of the degradation of N-end rule and ubiquitin-fusion degradation pathway substrates in mammalian cells.

Wójcik Cezary C Rowicka Maga M Kudlicki Andrzej A Nowis Dominika D McConnell Elizabeth E Kujawa Marek M DeMartino George N GN

Molecular biology of the cell 20060816 11

Valosin-containing protein (VCP; p97; cdc48 in yeast) is a hexameric ATPase of the AAA family (ATPases with multiple cellular activities) involved in multiple cellular functions, including degradation of proteins by the ubiquitin (Ub)-proteasome system (UPS). We examined the consequences of the reduction of VCP levels after RNA interference (RNAi) of VCP. A new stringent method of microarray analysis demonstrated that only four transcripts were nonspecifically affected by RNAi, whereas approxima ...[more]

PMID: 16914519

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