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Transcription profiling of Neuropeptide S Receptor 1-A (NPSR1-A) transfected human epithelial kidney cells stimulated with neuropeptide S to identify downstream targets

ABSTRACT: The purpose of this study was to identify downstream gene targets regulated by Neuropeptide S Receptor 1 (NPSR1) upon NPS stimulation. To do this we used human epithelial kidney (HEK)-293H cell lines stable transfected with NPSR1-A and stimulated with NPS for 6 hours. Two controls were used: NPS stimulated HEK-293H cells and unstimulated NPSR1-A cells.

ORGANISM(S): Homo sapiens

SUBMITTER: Tari Haahtela

PROVIDER: E-MEXP-829 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Downstream target genes of the neuropeptide S-NPSR1 pathway.

Vendelin Johanna J Bruce Sara S Holopainen Päivi P Pulkkinen Ville V Rytilä Paula P Pirskanen Asta A Rehn Marko M Laitinen Tarja T Laitinen Lauri A LA Haahtela Tari T Saarialho-Kere Ulpu U Laitinen Annika A Kere Juha J

Human molecular genetics 20060822 19

The neuropeptide S (NPS)-NPS receptor 1 (NPSR1) pathway has recently been implicated in the pathogenesis of asthma. The purpose of this study was to identify downstream gene targets regulated by NPSR1 upon NPS stimulation. A total of 104 genes were found significantly up-regulated and 42 down-regulated by microarray analysis 6 h after NPS administration. By Gene Ontology enrichment analysis, the categories 'cell proliferation', 'morphogenesis' and 'immune response' were among the most altered. A ...[more]

PMID: 16926187

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