Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of wild type and serrate-1 mutant Arabidopsis seedlings to investigate regulation of miRNA levels

ABSTRACT: microRNAs function as sequence-specific guides to regulate genes by post-transcriptional gene silencing. Many miRNAs are involved in the control plant development by regulating the accumulation of transcripts encoding transcription factors. This causes pleiotropic developmental phenotypes of mutants defective in miRNA accumulation, such as dcl1, hen1, hyl1 and ago1. The serrate-1 (se-1) mutant of Arabidopsis also exhibits a highly pleiotropic phenotype which overlaps with phenotypes of mutants defective in miRNA accumulation. It has been proposed that SE functions specifically in miRNA-mediated repression of the leaf polarity genes PHABULOSA and PHAVOLUTA. Microarray analysis of transcript levels in se-1 compared to wild-type however revealed upregulation of many genes known to be targets of miRNA regulation. We show that SE is a general regulator of miRNA levels probably controlling processing of pri-miRNA to miRNA.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: james hadfield

PROVIDER: E-MEXP-838 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

SERRATE: a new player on the plant microRNA scene.

Lobbes Dajana D Rallapalli Ghanasyam G Schmidt Dominik D DD Martin Cathie C Clarke Jonathan J

EMBO reports 20060915 10

MicroRNAs (miRNAs) function as sequence-specific guides that control gene expression by post-transcriptional gene silencing. Many miRNAs influence plant development by regulating the accumulation of transcripts that encode transcription factors. Mutants defective in miRNA accumulation, such as dcl1, hen1, hyl1 and ago1, have pleiotropic developmental phenotypes. The serrate-1 (se-1) mutant of Arabidopsis also shows a highly pleiotropic phenotype, which overlaps with the phenotypes of mutants def ...[more]

PMID: 16977334

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Dataset Information

Transcription profiling of wild type and serrate-1 mutant Arabidopsis seedlings to investigate regulation of miRNA levels

Publications

SERRATE: a new player on the plant microRNA scene.

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