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Transcription profiling time series of Sulfolobus acidocaldarius and Sulfolobus solfataricus after treatment with actinomycin D

ABSTRACT: Transcription inhibition by actinomycin D. RNA samples extracted at 3, 6, 9, 12, 15 min post actinomycin D. Each cDNA hybridised against reference cDNA from untreated culture.

INSTRUMENT(S): DNA Microarray Scanner BA [Agilent Technologies]

ORGANISM(S): Sulfolobus solfataricus

SUBMITTER: Rolf Bernander

PROVIDER: E-MEXP-894 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Global analysis of mRNA stability in the archaeon Sulfolobus.

Andersson Anders F AF Lundgren Magnus M Eriksson Stefan S Rosenlund Magnus M Bernander Rolf R Nilsson Peter P

Genome biology 20061026 10

<h4>Background</h4>Transcript half-lives differ between organisms, and between groups of genes within the same organism. The mechanisms underlying these differences are not clear, nor are the biochemical properties that determine the stability of a transcript. To address these issues, genome-wide mRNA decay studies have been conducted in eukaryotes and bacteria. In contrast, relatively little is known about RNA stability in the third domain of life, Archaea. Here, we present a microarray-based a ...[more]

PMID: 17067383

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