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Transcription profiling of human glioma cells treated with TGF-beta or an inhibitor of the TGF-beta Receptor I LY2109761

ABSTRACT: In order to identify the genes that are regulated by TGF-beta in glioma, we serum starved two glioma cell lines, U373MG and U87MG, for 16h and we treated them with vehicle,100pM TGF-beta, 2uM inhibitor of the TGF-beta Receptor I(TbRI)(LY2109761), or both 100pM TGF-beta plus 2uM TbRI for 3h. Then, cell were collected and total RNA was extracted.

ORGANISM(S): Homo sapiens

SUBMITTER: Avelina Tortosa

PROVIDER: E-MEXP-903 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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High TGFbeta-Smad activity confers poor prognosis in glioma patients and promotes cell proliferation depending on the methylation of the PDGF-B gene.

Bruna Alejandra A Darken Rachel S RS Rojo Federico F Ocaña Alberto A Peñuelas Silvia S Arias Alexandra A Paris Raquel R Tortosa Avelina A Mora Jaume J Baselga Jose J Seoane Joan J

Cancer cell 20070201 2

TGFbeta acts as a tumor suppressor in normal epithelial cells and early-stage tumors and becomes an oncogenic factor in advanced tumors. The molecular mechanisms involved in the malignant function of TGFbeta are not fully elucidated. We demonstrate that high TGFbeta-Smad activity is present in aggressive, highly proliferative gliomas and confers poor prognosis in patients with glioma. We discern the mechanisms and molecular determinants of the TGFbeta oncogenic response with a transcriptomic app ...[more]

PMID: 17292826

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