Transcription profiling of fibroblast-like synovial cells from normal adult horse following treatment with hyaluronan and challenged with lipopolysaccharide

ABSTRACT: Greater than 95% confluent (Day 0) fibroblast-like synovial cells from the hind limbs (pelvic limb) of normal adult horses were allocated in triplicate to one of 4 groups:

(group 1) unchallenged;
(group 2) LPS-challenged;
(group 3) LPS-challenged following pre-treatment and sustained treatment with lower (5 - 7.5 x 105 Da) molecular weight HA (Bioniche Animal Health, Pullman, WA) at 10 mg/ml;
(group 4) LPS-challenged following pre-treatment and sustained treatment with higher (3 x 106 Da) molecular weight HA (Pfizer Animal Health, New York, NY) at 5 mg/ml.

Microarray data were analyzed using dChip version 1.3 (Harvard University, Cambridge, MA). Array normalization was performed using the invariant set procedure; model-based expression indices (MBEI) were computed using only perfect match probes. Probe-set level data identified as array outliers by dChip were omitted and considered missing data in subsequent analyses of the log (base 2) MBEI values were then exported to BRB ArrayTools version 3.2.3 for further analysis (National Cancer Institute, Bethesda, MD). Paired t-tests compared gene expression between the unchallenged control group (group 1) and the LPS-challenged control group (group 2).
A two-way ANOVA compared gene expression among the LPS-challenged control group (group 2) and the two HA-treated groups (groups 3 and 4), blocking on horse. For the probe sets showing differential expression among the three LPS-challenged groups (p<0.005), pairwise comparisons were performed and p-values were adjusted by Holm's method. All tests involving gene expression data used a random variance model.

ORGANISM(S): Equus caballus

DISEASE(S): normal

SUBMITTER: Kelly Santangelo 

PROVIDER: E-MEXP-940 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress