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Transcription profiling of human CD8 T cells activated with CD3 or CD3-CD28 prior to retroviral transduction and different selection procedures (G418 or NGFR-MACS)n

ABSTRACT: effect of different T cell activations (CD3 and CD3-CD28) prior retroviral transduction and different selection procedures (G418 or NGFR-MACS)

ORGANISM(S): Homo sapiens

SUBMITTER: Christophe FERRAND

PROVIDER: E-MEXP-973 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome of retrovirally transduced CD8+ lymphocytes: influence of cell activation, transgene integration, and selection process.

Deschamps Marina M Robinet Eric E Certoux Jean Marie JM Mercier Patricia P Sauce Delphine D De Vos John J Montcuquet Nicolas N Bonyhadi Mark M Rème Thierry T Tiberghien Pierre P Ferrand Christophe C

Molecular immunology 20070910 4

A suicide gene introduced by retroviral means can allow in vivo control of alloreactivity mediated by donor gene-modified T cells (GMTC) after allogeneic hematopoietic stem cell transplantation. The present study establishes the transcriptomic profile of GMTC prepared according to the GMTC production process used in our clinical trial (activation/selection methods, CD3/NeoR), which was previously demonstrated to induce phenotypical and functional alterations. This transcriptomic profile was comp ...[more]

PMID: 17825913

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