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Transcription profiling of human regulatory and helper T cells following activation with anti-CD3 and anti-CD28 for 4 and 16 hours

ABSTRACT: Primary non-expanded human T cell populations were isolated by leukapheresis from 10 healthy donors. Out of each donor > 95% pure CD4-positive Th cell and >95% pure regulatory T cell populations were purified. Two affymetrix exon arrays were hybridized from each donor both populations as resting, non-activated samples. In addition, samples were hybridized from each donor and both populations activated by anti-CD3/anti-CD28 treatment for 4hours and 16 hours.

ORGANISM(S): Homo sapiens

SUBMITTER: Dr Detlev Mennerich

PROVIDER: E-TABM-779 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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