Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of pig postischaemic restituted intestinal mucosa after further ischaemic insult

ABSTRACT: Background: Restitution of the surface epithelium is a critical healing process early after ischaemic injury of the gut mucosa. Repeated episodes of intestinal ischaemia occur in many critically ill patients. This study evaluates the resistance of postischaemic restituted intestinal mucosa to a further ischaemic insult. Methods: The superior mesenteric artery (SMA) in pigs was cross-clamped for 1 hour, reperfused for 4 hours, cross-clamped once again for 1 hour, and finally reperfused for 3 hours. Intestinal injury was evaluated with morphometry. Intestinal permeability (from blood to lumen was assessed by transport of fluorescein isothiocyanate dextran (FD-4). Microarray and QRT-PCR analysis were performed.

INSTRUMENT(S): ScanArray Express HT [PerkinElmer]

ORGANISM(S): Sus scrofa

SUBMITTER: Vidar Beisvag

PROVIDER: E-MEXP-978 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Post-ischaemic restituted intestinal mucosa is more resistant to further ischaemia than normal mucosa in the pig.

Juel Ingebjørg S IS Solligård Erik E Tvedt Kåre E KE Skogvoll Eirik E Jynge Per P Beisvag Vidar V Erlandsen Sten Even SE Sandvik Arne K AK Aadahl Petter P Grønbech Jon Erik JE

Scandinavian journal of clinical and laboratory investigation 20071121 2

<h4>Objective</h4>Ischaemic preconditioning may protect the intestine from subsequent prolonged ischaemia. This study evaluates whether a much longer initial ischaemia, encountered clinically, may modify intestinal resistance to further ischaemia in a pig model.<h4>Material and methods</h4>After cross-clamping of the superior mesenteric artery for 1 h, the intestine was either reperfused for 8 h or a second cross-clamping for 1 h was performed at 4 h of reperfusion. Based on microarray analysis ...[more]

PMID: 17852833

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Dataset Information

Transcription profiling of pig postischaemic restituted intestinal mucosa after further ischaemic insult

Publications

Post-ischaemic restituted intestinal mucosa is more resistant to further ischaemia than normal mucosa in the pig.

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