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Chromatin immunoprecipitation of human breast cancer cell line MCF-7 treated with 17beta-estradiol

ABSTRACT: ChIP-DSL experiments using ER alpha antibody on v1 20k promoter array

INSTRUMENT(S): GenePix 4000B [Axon Instruments]

ORGANISM(S): Homo sapiens

SUBMITTER: Kasey Hutt

PROVIDER: E-MEXP-984 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Sensitive ChIP-DSL technology reveals an extensive estrogen receptor alpha-binding program on human gene promoters.

Kwon Young-Soo YS Garcia-Bassets Ivan I Hutt Kasey R KR Cheng Christine S CS Jin Mingjie M Liu Dongyan D Benner Chris C Wang Dong D Ye Zhen Z Bibikova Marina M Fan Jian-Bing JB Duan Lingxun L Glass Christopher K CK Rosenfeld Michael G MG Fu Xiang-Dong XD

Proceedings of the National Academy of Sciences of the United States of America 20070314 12

ChIP coupled with microarray provides a powerful tool to determine in vivo binding profiling of transcription factors to deduce regulatory circuitries in mammalian cells. Aiming at improving the specificity and sensitivity of such analysis, we developed a new technology called ChIP-DSL using the DNA selection and ligation (DSL) strategy, permitting robust analysis with much reduced materials compared with standard procedures. We profiled general and sequence-specific DNA binding transcription fa ...[more]

PMID: 17360330

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