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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of mouse embryonic stem cells expressing the transcription factor NGN3 for 12 hours, 24 hours and 48 hours

ABSTRACT: A mouse embryonic stem cell line was generated which stably expressed the ngn3 transcription factor under the control of the Tet-On inducible system using knock-ins in the ROSA26 and the HPRT loci. The undifferentiated mouse embryonic stem cells were then differentiated into Embryoid Bodies in suspension culture and were either treated with Doxycycline to induce NGN3 expression or left untreated as a contol. Cells were harvested at 12 hours, 24 hours and 48 hours.

ORGANISM(S): Mus musculus

SUBMITTER: Charles Angela

PROVIDER: E-MIMR-461 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A shift from reversible to irreversible X inactivation is triggered during ES cell differentiation.

Wutz A A Jaenisch R R

Molecular cell 20000401 4

Xist is required for X inactivation. To study the initiation of X inactivation, we have generated a full-length mouse Xist cDNA transgene and an inducible expression system facilitating controlled Xist expression in ES cells and differentiated cultures. In ES cells, transgenic Xist RNA was stable and caused long-range transcriptional repression in cis. Repression was reversible and dependent on continued Xist expression in ES cells and early ES cell differentiation. By 72 hr of differentiation, ...[more]

PMID: 10882105

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