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Transcription profiling of rat primary cardiac myocytes treated with endothelin-1, cycloheximide, azekenpaullone or combinations of these before extraction of total or polysomal RNA

ABSTRACT: Ventricles were removed from 2-day Sprague_Dawley rat litters from which primary cardiac myocytes (>95%) were isolated. Myocytes were either untreated or exposed to either endothelin-1 for 30, 60 or 120 minutes, cycloheximide for 70 or 130 minutes, endothelin-1 for 10 minutes followed by cycloheximide for 70 minutes, azekenpaullone for 60 minutes before extraction of total or polysomal (actively transcribed) RNA. Labelled cRNA was hybridised to Affymetrix Rat 230 2.0 arrays.

ORGANISM(S): Rattus rattus

SUBMITTER: Tom Bradley

PROVIDER: E-MIMR-681 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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