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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human chondrocytes from patients with osteosarcoma or osteomyelitis, cultured in either 1% or 20% oxygen for one or four days

ABSTRACT: Cartilage was taken from the femoral condyle and tibial plateau from eight patients, cut into small pieces and digested overnight with Collagenase Type 2. The resultant chondrocytes were put through a cell strainer, pelleted and resuspended in fresh media and cultured for one week in 20% oxygen. The culture was split into five sub-cultures, one of which was immediately frozen; the remainder were allowed to recover overnight. Cells were subsequently grown at either 1% oxygen (hypoxia simulation) or 20% oxygen (normoxia simulation) for either one or four days before RNA extraction. Labelled RNA was then hybridised to Affymetrix Human U133 plus 2.0 arrays.

ORGANISM(S): Homo sapiens

DISEASE(S): osteosarcoma

SUBMITTER: Jayne Dennis

PROVIDER: E-MIMR-644 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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